Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans

Hisako Yamagishi, Deirdre H. Fitzgerald, Tin Sein, Thomas J. Walsh, Brian C. O'Connell

Research output: Contribution to journalArticle

Abstract

Antifungal activity of histatin 3 against two Candida albicans clinical isolates was determined in assays containing rabbit submandibular gland saliva. Histatin 3 inhibited the cell growth and germination of both isolates dose-dependently (10-100 μg ml-1) with maximum inhibition occurring after 60 min incubation. Adding fresh histatin 3 after 60 min caused further reduction in the viable cell count. Higher histatin 3 concentrations (50-100 μg ml-1) and prolonged exposure to peptide were required to inhibit germination. Histatin 3 was rapidly degraded in rabbit submandibular gland saliva and this may explain why fresh addition of histatin 3 increases candidacidal activity.

Original languageEnglish (US)
Pages (from-to)207-212
Number of pages6
JournalFEMS Microbiology Letters
Volume244
Issue number1
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

Fingerprint

Histatins
Candida albicans
Saliva
Submandibular Gland
Germination
Rabbits
Cell Count
Peptides
Growth

Keywords

  • Antifungal
  • Candida
  • Histatin
  • Histatin degradation
  • Rabbit submandibular gland saliva
  • Saliva

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans. / Yamagishi, Hisako; Fitzgerald, Deirdre H.; Sein, Tin; Walsh, Thomas J.; O'Connell, Brian C.

In: FEMS Microbiology Letters, Vol. 244, No. 1, 01.03.2005, p. 207-212.

Research output: Contribution to journalArticle

Yamagishi, Hisako ; Fitzgerald, Deirdre H. ; Sein, Tin ; Walsh, Thomas J. ; O'Connell, Brian C. / Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans. In: FEMS Microbiology Letters. 2005 ; Vol. 244, No. 1. pp. 207-212.
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