Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans

Hisako Yamagishi, Deirdre H. Fitzgerald, Tin Sein, Thomas J. Walsh, Brian C. O'Connell

Research output: Contribution to journalArticlepeer-review

Abstract

Antifungal activity of histatin 3 against two Candida albicans clinical isolates was determined in assays containing rabbit submandibular gland saliva. Histatin 3 inhibited the cell growth and germination of both isolates dose-dependently (10-100 μg ml-1) with maximum inhibition occurring after 60 min incubation. Adding fresh histatin 3 after 60 min caused further reduction in the viable cell count. Higher histatin 3 concentrations (50-100 μg ml-1) and prolonged exposure to peptide were required to inhibit germination. Histatin 3 was rapidly degraded in rabbit submandibular gland saliva and this may explain why fresh addition of histatin 3 increases candidacidal activity.

Original languageEnglish (US)
Pages (from-to)207-212
Number of pages6
JournalFEMS microbiology letters
Volume244
Issue number1
DOIs
StatePublished - Mar 1 2005

Keywords

  • Antifungal
  • Candida
  • Histatin
  • Histatin degradation
  • Rabbit submandibular gland saliva
  • Saliva

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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