Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans

Hisako Yamagishi; Deirdre H. Fitzgerald; Tin Sein; Thomas J. Walsh; Brian C. O'Connell

doi:10.1016/j.femsle.2005.01.045

Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans

Hisako Yamagishi, Deirdre H. Fitzgerald, Tin Sein, Thomas J. Walsh, Brian C. O'Connell

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Antifungal activity of histatin 3 against two Candida albicans clinical isolates was determined in assays containing rabbit submandibular gland saliva. Histatin 3 inhibited the cell growth and germination of both isolates dose-dependently (10-100 μg ml^-1) with maximum inhibition occurring after 60 min incubation. Adding fresh histatin 3 after 60 min caused further reduction in the viable cell count. Higher histatin 3 concentrations (50-100 μg ml^-1) and prolonged exposure to peptide were required to inhibit germination. Histatin 3 was rapidly degraded in rabbit submandibular gland saliva and this may explain why fresh addition of histatin 3 increases candidacidal activity.

Original language	English (US)
Pages (from-to)	207-212
Number of pages	6
Journal	FEMS microbiology letters
Volume	244
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.01.045
State	Published - Mar 1 2005
Externally published	Yes

Keywords

Antifungal
Candida
Histatin
Histatin degradation
Rabbit submandibular gland saliva
Saliva

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/j.femsle.2005.01.045

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title = "Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans",

abstract = "Antifungal activity of histatin 3 against two Candida albicans clinical isolates was determined in assays containing rabbit submandibular gland saliva. Histatin 3 inhibited the cell growth and germination of both isolates dose-dependently (10-100 μg ml-1) with maximum inhibition occurring after 60 min incubation. Adding fresh histatin 3 after 60 min caused further reduction in the viable cell count. Higher histatin 3 concentrations (50-100 μg ml-1) and prolonged exposure to peptide were required to inhibit germination. Histatin 3 was rapidly degraded in rabbit submandibular gland saliva and this may explain why fresh addition of histatin 3 increases candidacidal activity.",

keywords = "Antifungal, Candida, Histatin, Histatin degradation, Rabbit submandibular gland saliva, Saliva",

author = "Hisako Yamagishi and Fitzgerald, {Deirdre H.} and Tin Sein and Walsh, {Thomas J.} and O'Connell, {Brian C.}",

note = "Funding Information: The authors thank the National Institutes of Health (NIH) for financial support.",

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AU - Yamagishi, Hisako

AU - Fitzgerald, Deirdre H.

AU - Sein, Tin

AU - Walsh, Thomas J.

AU - O'Connell, Brian C.

N1 - Funding Information: The authors thank the National Institutes of Health (NIH) for financial support.

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AB - Antifungal activity of histatin 3 against two Candida albicans clinical isolates was determined in assays containing rabbit submandibular gland saliva. Histatin 3 inhibited the cell growth and germination of both isolates dose-dependently (10-100 μg ml-1) with maximum inhibition occurring after 60 min incubation. Adding fresh histatin 3 after 60 min caused further reduction in the viable cell count. Higher histatin 3 concentrations (50-100 μg ml-1) and prolonged exposure to peptide were required to inhibit germination. Histatin 3 was rapidly degraded in rabbit submandibular gland saliva and this may explain why fresh addition of histatin 3 increases candidacidal activity.

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KW - Histatin

KW - Histatin degradation

KW - Rabbit submandibular gland saliva

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Saliva affects the antifungal activity of exogenously added histatin 3 towards Candida albicans

Abstract

Keywords

ASJC Scopus subject areas

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