Safety and immunogenicity of an oral, inactivated enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine in Bangladeshi adults and children

Firdausi Qadri, Christine Wennerås, Firoz Ahmed, Muhammad Asaduzzaman, Debashish Saha, M. J. Albert, R. B. Sack, Ann Mari Svennerholm

Research output: Contribution to journalArticle

Abstract

We have compared the B cell responses evoked in Bangladeshi, adults (n=11, median age 25 years) and children (n=21, median age 4.5 years), 7 days after intake of each of two doses of an oral, inactivated enterotoxigenic Escherichia coli (ETEC) vaccine composed of formalin-killed ETEC strains expressing the colonization factors, CFA/I, CFA/II and CFA/IV together with 1 mg of recombinant cholera toxin B-subunit (rCTB). The vaccine was well tolerated and only gave rise to negligible side effects. Peak antibody-secreting cell (ASC) response of the IgA isotype were seen 7 days after the first dose of the vaccine. The ASC responses to the different colonization factors (CFs) increased from a 29- to 46-fold (responder frequency 90-100%) in the adults and 13- to 24-fold (responder frequency 67-90%) in the children. The IgA-ASC response to rCTB also peaked after the first dose in the adults (426-fold, responder frequency 100%) and the children (46-fold, responder frequency 95%). Increased IgA antibody levels against CFA/I as well as IgA and IgG antibody levels to rCTB were seen in plasma after immunisation. About 86% of the children and 80% of the adults responded with faecal antibodies to rCTB, whereas about 67% of both groups responded to CFA/I.These results show that a single dose of the ETEC vaccine may elicit significant mucosal immune responses in both children and adults residing in an ETEC-endemic country such as Bangladesh. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)2704-2712
Number of pages9
JournalVaccine
Volume18
Issue number24
StatePublished - Jun 1 2000
Externally publishedYes

Fingerprint

Enterotoxigenic Escherichia coli
enterotoxigenic Escherichia coli
Subunit Vaccines
cholera toxin
subunit vaccines
Cholera Toxin
mouth
Antibody-Producing Cells
Immunoglobulin A
immune response
Safety
Escherichia coli Vaccines
antibodies
vaccines
Antibodies
dosage
Vaccines
Mucosal Immunity
Bangladesh
mucosal immunity

Keywords

  • Antibody-secreting cells
  • Eenterotoxigenic Escherichia coli
  • Oral inactivated ETEC vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Qadri, F., Wennerås, C., Ahmed, F., Asaduzzaman, M., Saha, D., Albert, M. J., ... Svennerholm, A. M. (2000). Safety and immunogenicity of an oral, inactivated enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine in Bangladeshi adults and children. Vaccine, 18(24), 2704-2712.

Safety and immunogenicity of an oral, inactivated enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine in Bangladeshi adults and children. / Qadri, Firdausi; Wennerås, Christine; Ahmed, Firoz; Asaduzzaman, Muhammad; Saha, Debashish; Albert, M. J.; Sack, R. B.; Svennerholm, Ann Mari.

In: Vaccine, Vol. 18, No. 24, 01.06.2000, p. 2704-2712.

Research output: Contribution to journalArticle

Qadri, F, Wennerås, C, Ahmed, F, Asaduzzaman, M, Saha, D, Albert, MJ, Sack, RB & Svennerholm, AM 2000, 'Safety and immunogenicity of an oral, inactivated enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine in Bangladeshi adults and children', Vaccine, vol. 18, no. 24, pp. 2704-2712.
Qadri, Firdausi ; Wennerås, Christine ; Ahmed, Firoz ; Asaduzzaman, Muhammad ; Saha, Debashish ; Albert, M. J. ; Sack, R. B. ; Svennerholm, Ann Mari. / Safety and immunogenicity of an oral, inactivated enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine in Bangladeshi adults and children. In: Vaccine. 2000 ; Vol. 18, No. 24. pp. 2704-2712.
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abstract = "We have compared the B cell responses evoked in Bangladeshi, adults (n=11, median age 25 years) and children (n=21, median age 4.5 years), 7 days after intake of each of two doses of an oral, inactivated enterotoxigenic Escherichia coli (ETEC) vaccine composed of formalin-killed ETEC strains expressing the colonization factors, CFA/I, CFA/II and CFA/IV together with 1 mg of recombinant cholera toxin B-subunit (rCTB). The vaccine was well tolerated and only gave rise to negligible side effects. Peak antibody-secreting cell (ASC) response of the IgA isotype were seen 7 days after the first dose of the vaccine. The ASC responses to the different colonization factors (CFs) increased from a 29- to 46-fold (responder frequency 90-100{\%}) in the adults and 13- to 24-fold (responder frequency 67-90{\%}) in the children. The IgA-ASC response to rCTB also peaked after the first dose in the adults (426-fold, responder frequency 100{\%}) and the children (46-fold, responder frequency 95{\%}). Increased IgA antibody levels against CFA/I as well as IgA and IgG antibody levels to rCTB were seen in plasma after immunisation. About 86{\%} of the children and 80{\%} of the adults responded with faecal antibodies to rCTB, whereas about 67{\%} of both groups responded to CFA/I.These results show that a single dose of the ETEC vaccine may elicit significant mucosal immune responses in both children and adults residing in an ETEC-endemic country such as Bangladesh. Copyright (C) 2000 Elsevier Science Ltd.",
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