Saccharomyces cerevisiae ISU1 and ISU2: Members of a well-conserved gene family for iron-sulfur cluster assembly

Stacey A. Garland, Kevin Hoff, Larry E. Vickery, Valeria L Culotta

Research output: Contribution to journalArticle

Abstract

Recent studies in bacteria and eukaryotes have led to the identification of several new genes implicated in the biogenesis of iron-sulfur (Fe/S) cluster-containing proteins. This report focuses on two genes of bakers yeast Saccharomyces cerevisiae, ISU1 and ISU2, which encode homologues to bacterial IscU and NifU, potential iron-binding or cluster-assembly proteins. As with other yeast genes implicated in Fe/S protein assembly, deletion of either ISU1 or ISU2 results in increased accumulation of iron within the mitochondria, loss of activity of the [4Fe-4S] aconitase enzyme, and suppression of oxidative damage in cells lacking cytosolic copper/zinc superoxide dismutase. Both genes are induced in strains expressing an activated allele of Aft1p, the iron-sensing transcription factor, suggesting that they are regulated by the iron status of the cell. Immunoblotting studies using an antibody directed against Escherichia coli IscU reveal that both Isu1p and Isu2p are localized primarily in the mitochondria and that Isu1p is the predominant form expressed under all growth conditions tested. The possible role of the Isu proteins in the assembly and/or repair of Fe/S clusters is discussed.

Original languageEnglish (US)
Pages (from-to)897-907
Number of pages11
JournalJournal of Molecular Biology
Volume294
Issue number4
DOIs
StatePublished - Dec 10 1999

Fingerprint

Sulfur
Saccharomyces cerevisiae
Iron
Genes
Mitochondria
Aconitate Hydratase
Proteins
Protein S
Eukaryota
Immunoblotting
Superoxide Dismutase
Zinc
Copper
Transcription Factors
Yeasts
Alleles
Escherichia coli
Bacteria
Antibodies
Enzymes

Keywords

  • Iron
  • iscU
  • ISU
  • Superoxide
  • Yeast

ASJC Scopus subject areas

  • Virology

Cite this

Saccharomyces cerevisiae ISU1 and ISU2 : Members of a well-conserved gene family for iron-sulfur cluster assembly. / Garland, Stacey A.; Hoff, Kevin; Vickery, Larry E.; Culotta, Valeria L.

In: Journal of Molecular Biology, Vol. 294, No. 4, 10.12.1999, p. 897-907.

Research output: Contribution to journalArticle

@article{87f66121273341308bdca25d0113708c,
title = "Saccharomyces cerevisiae ISU1 and ISU2: Members of a well-conserved gene family for iron-sulfur cluster assembly",
abstract = "Recent studies in bacteria and eukaryotes have led to the identification of several new genes implicated in the biogenesis of iron-sulfur (Fe/S) cluster-containing proteins. This report focuses on two genes of bakers yeast Saccharomyces cerevisiae, ISU1 and ISU2, which encode homologues to bacterial IscU and NifU, potential iron-binding or cluster-assembly proteins. As with other yeast genes implicated in Fe/S protein assembly, deletion of either ISU1 or ISU2 results in increased accumulation of iron within the mitochondria, loss of activity of the [4Fe-4S] aconitase enzyme, and suppression of oxidative damage in cells lacking cytosolic copper/zinc superoxide dismutase. Both genes are induced in strains expressing an activated allele of Aft1p, the iron-sensing transcription factor, suggesting that they are regulated by the iron status of the cell. Immunoblotting studies using an antibody directed against Escherichia coli IscU reveal that both Isu1p and Isu2p are localized primarily in the mitochondria and that Isu1p is the predominant form expressed under all growth conditions tested. The possible role of the Isu proteins in the assembly and/or repair of Fe/S clusters is discussed.",
keywords = "Iron, iscU, ISU, Superoxide, Yeast",
author = "Garland, {Stacey A.} and Kevin Hoff and Vickery, {Larry E.} and Culotta, {Valeria L}",
year = "1999",
month = "12",
day = "10",
doi = "10.1006/jmbi.1999.3294",
language = "English (US)",
volume = "294",
pages = "897--907",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Saccharomyces cerevisiae ISU1 and ISU2

T2 - Members of a well-conserved gene family for iron-sulfur cluster assembly

AU - Garland, Stacey A.

AU - Hoff, Kevin

AU - Vickery, Larry E.

AU - Culotta, Valeria L

PY - 1999/12/10

Y1 - 1999/12/10

N2 - Recent studies in bacteria and eukaryotes have led to the identification of several new genes implicated in the biogenesis of iron-sulfur (Fe/S) cluster-containing proteins. This report focuses on two genes of bakers yeast Saccharomyces cerevisiae, ISU1 and ISU2, which encode homologues to bacterial IscU and NifU, potential iron-binding or cluster-assembly proteins. As with other yeast genes implicated in Fe/S protein assembly, deletion of either ISU1 or ISU2 results in increased accumulation of iron within the mitochondria, loss of activity of the [4Fe-4S] aconitase enzyme, and suppression of oxidative damage in cells lacking cytosolic copper/zinc superoxide dismutase. Both genes are induced in strains expressing an activated allele of Aft1p, the iron-sensing transcription factor, suggesting that they are regulated by the iron status of the cell. Immunoblotting studies using an antibody directed against Escherichia coli IscU reveal that both Isu1p and Isu2p are localized primarily in the mitochondria and that Isu1p is the predominant form expressed under all growth conditions tested. The possible role of the Isu proteins in the assembly and/or repair of Fe/S clusters is discussed.

AB - Recent studies in bacteria and eukaryotes have led to the identification of several new genes implicated in the biogenesis of iron-sulfur (Fe/S) cluster-containing proteins. This report focuses on two genes of bakers yeast Saccharomyces cerevisiae, ISU1 and ISU2, which encode homologues to bacterial IscU and NifU, potential iron-binding or cluster-assembly proteins. As with other yeast genes implicated in Fe/S protein assembly, deletion of either ISU1 or ISU2 results in increased accumulation of iron within the mitochondria, loss of activity of the [4Fe-4S] aconitase enzyme, and suppression of oxidative damage in cells lacking cytosolic copper/zinc superoxide dismutase. Both genes are induced in strains expressing an activated allele of Aft1p, the iron-sensing transcription factor, suggesting that they are regulated by the iron status of the cell. Immunoblotting studies using an antibody directed against Escherichia coli IscU reveal that both Isu1p and Isu2p are localized primarily in the mitochondria and that Isu1p is the predominant form expressed under all growth conditions tested. The possible role of the Isu proteins in the assembly and/or repair of Fe/S clusters is discussed.

KW - Iron

KW - iscU

KW - ISU

KW - Superoxide

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=0033544704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033544704&partnerID=8YFLogxK

U2 - 10.1006/jmbi.1999.3294

DO - 10.1006/jmbi.1999.3294

M3 - Article

C2 - 10588895

AN - SCOPUS:0033544704

VL - 294

SP - 897

EP - 907

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -