S. pombe replication protein Cdc18 (Cdc6) interacts with Swi6 (HP1) heterochromatin protein: Region specific effects and replication timing in the centromere

Pao Chen Li, Louise Chretien, Jocelyn Côté, Thomas J. Kelly, Susan L. Forsburg

Research output: Contribution to journalArticle

Abstract

Heterochromatin in S. pombe is associated with gene silencing at telomeres, the mating locus and centromeres. The compact heterochromatin structure raises the question how it unpacks and reforms during DNA replication. We show that the essential DNA replication factor Cdc18 (CDC6) associates with heterochromatin protein 1 (Swi6) in vivo and in vitro. Biochemical mapping and mutational analysis of the association domains show that the N-terminus of Cdc18 interacts with the chromoshadow domain of Swi6. Mutations in Swi6 that disrupt this interaction disrupt silencing and delay replication in the centromere. A mutation cdc18-I43A that reduces Cdc18 association with Swi6 has no silencing defect at the centromere, but changes Swi6 distribution and accelerates the timing of centromere replication. We suggest that fine tuning of Swi6 association at replication origins is important for negative as well as positive control of replication initiation.

Original languageEnglish (US)
Pages (from-to)323-336
Number of pages14
JournalCell Cycle
Volume10
Issue number2
DOIs
StatePublished - Jan 15 2011
Externally publishedYes

Keywords

  • Cdc18
  • Centromere
  • DNA replication
  • Fission yeast
  • Swi6

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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