Rv2190c, an NlpC/P60 Family Protein, Is Required for Full Virulence of Mycobacterium tuberculosis

Geetha Parthasarathy, Shichun Lun, Haidan Guo, Nicole Ammerman, Deborah E. Geiman, William Ramses Bishai

Research output: Contribution to journalArticle

Abstract

Mycobacterium tuberculosis, the etiologic agent of tuberculosis (TB) possesses at least five genes predicted to encode proteins with NlpC/P60 hydrolase domains, including the relatively uncharacterized Rv2190c. As NlpC/P60 domain-containing proteins are associated with diverse roles in bacterial physiology, our objective was to characterize Rv2190c in M. tuberculosis growth and virulence. Our data indicate that lack of Rv2190c is associated with impaired growth, both in vitro and during an in vivo mouse model of TB. These growth defects are associated with altered colony morphology and phthiocerol dimycocerosate levels, indicating that Rv2190c is involved in cell wall maintenance and composition. In addition, we have demonstrated that Rv2190c is expressed during active growth phase and that its protein product is immunogenic during infection. Our findings have significant implications, both for better understanding the role of Rv2190c in M. tuberculosis biology and also for translational developments.

Original languageEnglish (US)
Article numbere43429
JournalPLoS One
Volume7
Issue number8
DOIs
StatePublished - Aug 31 2012

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Mycobacterium tuberculosis
Virulence
virulence
tuberculosis
Growth
microbial physiology
Tuberculosis
Proteins
Bacterial Physiological Phenomena
proteins
protein products
hydrolases
Physiology
Hydrolases
animal models
cell walls
Cell Wall
Biological Sciences
Genes
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rv2190c, an NlpC/P60 Family Protein, Is Required for Full Virulence of Mycobacterium tuberculosis. / Parthasarathy, Geetha; Lun, Shichun; Guo, Haidan; Ammerman, Nicole; Geiman, Deborah E.; Bishai, William Ramses.

In: PLoS One, Vol. 7, No. 8, e43429, 31.08.2012.

Research output: Contribution to journalArticle

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