Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis

Lucie Rochard, Stefanie D. Monica, Irving T.C. Ling, Yawei Kong, Sara Roberson, Richard Harland, Marnie Halpern, Eric C. Liao

Research output: Contribution to journalArticle

Abstract

The Wnt signaling pathway is crucial for tissue morphogenesis, participating in cellular behavior changes, notably during the process of convergent-extension. Interactions between Wnt-secreting and receiving cells during convergent-extension remain elusive. We investigated the role and genetic interactions of Wnt ligands and their trafficking factors Wls, Gpc4 and Frzb in the context of palate morphogenesis in zebrafish. We describe that the chaperon Wls and its ligands Wnt9a and Wnt5b are expressed in the ectoderm, whereas juxtaposed chondrocytes express Frzb and Gpc4. Using wls, gpc4, frzb, wnt9a and wnt5b mutants, we genetically dissected the Wnt signals operating between secreting ectoderm and receiving chondrocytes. Our analysis delineates that non-canonical Wnt signaling is required for cell intercalation, and that wnt5b and wnt9a are required for palate extension in the anteroposterior and transverse axes, respectively.

Original languageEnglish (US)
Pages (from-to)2541-2547
Number of pages7
JournalDevelopment (Cambridge)
Volume143
Issue number14
DOIs
StatePublished - Jul 15 2016

Keywords

  • Convergent-extension
  • Morphogenesis
  • Palate
  • Wnt
  • Zebrabow

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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