Role of the RNA-binding protein HuR in colon carcinogenesis

Isabel López De Silanes, Jinshui Fan, Xiaoling Yang, Alan B. Zonderman, Olga Potapova, Ellen S. Pizer, Myriam Gorospe

Research output: Contribution to journalArticle

Abstract

Immunohistochemical analysis of paired tumor and normal tissue specimens revealed that the expression and cytoplasmic abundance of the RNA-binding protein HuR increased with malignancy, particularly in colon carcinomas. Interventions to modulate HuR expression in human RKO colon cancer cells altered gene expression profiles and identified β-catenin mRNA as a novel HuR target. Subcutaneous injection of HuR-overexpressing RKO cells into nude mice produced significantly larger tumors than those arising from control populations; conversely, RKO cells expressing reduced HuR through small interference RNA- or antisense HuR-based approaches developed significantly more slowly. We propose that HuR-regulated target mRNA expression contributes to colon cancer growth. Our results suggest a pivotal function for HuR in colon carcinogenesis.

Original languageEnglish (US)
Pages (from-to)7146-7154
Number of pages9
JournalOncogene
Volume22
Issue number46
DOIs
StatePublished - Oct 16 2003
Externally publishedYes

Fingerprint

RNA-Binding Proteins
Colon
Carcinogenesis
Colonic Neoplasms
Antisense RNA
Catenins
Neoplasms
Messenger RNA
Subcutaneous Injections
RNA Interference
Transcriptome
Nude Mice
Carcinoma
Growth
Population

Keywords

  • Carcinogenesis
  • Colon cancer
  • ELAV
  • HuR
  • mRNA turnover

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

López De Silanes, I., Fan, J., Yang, X., Zonderman, A. B., Potapova, O., Pizer, E. S., & Gorospe, M. (2003). Role of the RNA-binding protein HuR in colon carcinogenesis. Oncogene, 22(46), 7146-7154. https://doi.org/10.1038/sj.onc.1206862

Role of the RNA-binding protein HuR in colon carcinogenesis. / López De Silanes, Isabel; Fan, Jinshui; Yang, Xiaoling; Zonderman, Alan B.; Potapova, Olga; Pizer, Ellen S.; Gorospe, Myriam.

In: Oncogene, Vol. 22, No. 46, 16.10.2003, p. 7146-7154.

Research output: Contribution to journalArticle

López De Silanes, I, Fan, J, Yang, X, Zonderman, AB, Potapova, O, Pizer, ES & Gorospe, M 2003, 'Role of the RNA-binding protein HuR in colon carcinogenesis', Oncogene, vol. 22, no. 46, pp. 7146-7154. https://doi.org/10.1038/sj.onc.1206862
López De Silanes I, Fan J, Yang X, Zonderman AB, Potapova O, Pizer ES et al. Role of the RNA-binding protein HuR in colon carcinogenesis. Oncogene. 2003 Oct 16;22(46):7146-7154. https://doi.org/10.1038/sj.onc.1206862
López De Silanes, Isabel ; Fan, Jinshui ; Yang, Xiaoling ; Zonderman, Alan B. ; Potapova, Olga ; Pizer, Ellen S. ; Gorospe, Myriam. / Role of the RNA-binding protein HuR in colon carcinogenesis. In: Oncogene. 2003 ; Vol. 22, No. 46. pp. 7146-7154.
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