Abstract
Immunohistochemical analysis of paired tumor and normal tissue specimens revealed that the expression and cytoplasmic abundance of the RNA-binding protein HuR increased with malignancy, particularly in colon carcinomas. Interventions to modulate HuR expression in human RKO colon cancer cells altered gene expression profiles and identified β-catenin mRNA as a novel HuR target. Subcutaneous injection of HuR-overexpressing RKO cells into nude mice produced significantly larger tumors than those arising from control populations; conversely, RKO cells expressing reduced HuR through small interference RNA- or antisense HuR-based approaches developed significantly more slowly. We propose that HuR-regulated target mRNA expression contributes to colon cancer growth. Our results suggest a pivotal function for HuR in colon carcinogenesis.
Original language | English (US) |
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Pages (from-to) | 7146-7154 |
Number of pages | 9 |
Journal | Oncogene |
Volume | 22 |
Issue number | 46 |
DOIs | |
State | Published - Oct 16 2003 |
Externally published | Yes |
Keywords
- Carcinogenesis
- Colon cancer
- ELAV
- HuR
- mRNA turnover
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research