@article{dd35730c12d2426fa9f1fd58ee1599a6,
title = "Role of the Internal Limiting Membrane in Structural Engraftment and Topographic Spacing of Transplanted Human Stem Cell-Derived Retinal Ganglion Cells",
abstract = "In this article, Johnson and colleagues co-cultured human embryonic stem cell-derived retinal ganglion cells (RGCs) on the surface of adult organotypic retinal explants. The authors showed that RGCs spontaneously clustered and extended neurites that were excluded from the retinal parenchyma, but that internal limiting membrane (ILM) digestion permitted robust neurite engraftment with little toxicity, thereby identifying the ILM as a barrier to transplanted RGC retinal integration.",
keywords = "cellular interactions, dendrite, engraftment, glaucoma, migration, optic nerve regeneration, optic neuropathy, regenerative medicine, retinal ganglion cell, transplantation",
author = "Zhang, {Kevin Y.} and Caitlyn Tuffy and Mertz, {Joseph L.} and Sarah Quillen and Laurence Wechsler and Quigley, {Harry A.} and Zack, {Donald J.} and Johnson, {Thomas V.}",
note = "Funding Information: This work was funded by the National Eye Institute (K12EY015025 [T.J.]; K08EY031801 [T.J.]; R01EY002120 [H.Q.]; P30EY001765 [D.Z.]); The ARVO David L Epstein Award (H.Q. and T.J.); Research to Prevent Blindness (Career Development Award [T.J.] and Unrestricted Grant Funding [Wilmer Eye Institute]); The American Glaucoma Society (Mentoring for the Advancement of Physician Scientists Grant [T.J.]); The Johns Hopkins Physician Scientist Training Program (Pilot Grant [T.J.]); and generous gifts from the Guerrieri Family Foundation, the Gilbert Family Foundation, and the Marion & Robert Rosenthal Family Foundation. The authors are grateful to Dr. Alex Kolodkin (Department of Neuroscience, Johns Hopkins University) for critical evaluation of the manuscript. Funding Information: This work was funded by the National Eye Institute ( K12EY015025 [T.J.]; K08EY031801 [T.J.]; R01EY002120 [H.Q.]; P30EY001765 [D.Z.]); The ARVO David L Epstein Award (H.Q. and T.J.); Research to Prevent Blindness (Career Development Award [T.J.] and Unrestricted Grant Funding [Wilmer Eye Institute]); The American Glaucoma Society (Mentoring for the Advancement of Physician Scientists Grant [T.J.]); The Johns Hopkins Physician Scientist Training Program (Pilot Grant [T.J.]); and generous gifts from the Guerrieri Family Foundation , the Gilbert Family Foundation , and the Marion & Robert Rosenthal Family Foundation . The authors are grateful to Dr. Alex Kolodkin (Department of Neuroscience, Johns Hopkins University) for critical evaluation of the manuscript. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2021",
month = jan,
day = "12",
doi = "10.1016/j.stemcr.2020.12.001",
language = "English (US)",
volume = "16",
pages = "149--167",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "1",
}