Role of the Internal Limiting Membrane in Structural Engraftment and Topographic Spacing of Transplanted Human Stem Cell-Derived Retinal Ganglion Cells

Kevin Y. Zhang, Caitlyn Tuffy, Joseph L. Mertz, Sarah Quillen, Laurence Wechsler, Harry A. Quigley, Donald J. Zack, Thomas V. Johnson

Research output: Contribution to journalArticlepeer-review

Abstract

In this article, Johnson and colleagues co-cultured human embryonic stem cell-derived retinal ganglion cells (RGCs) on the surface of adult organotypic retinal explants. The authors showed that RGCs spontaneously clustered and extended neurites that were excluded from the retinal parenchyma, but that internal limiting membrane (ILM) digestion permitted robust neurite engraftment with little toxicity, thereby identifying the ILM as a barrier to transplanted RGC retinal integration.

Original languageEnglish (US)
Pages (from-to)149-167
Number of pages19
JournalStem Cell Reports
Volume16
Issue number1
DOIs
StatePublished - Jan 12 2021

Keywords

  • cellular interactions
  • dendrite
  • engraftment
  • glaucoma
  • migration
  • optic nerve regeneration
  • optic neuropathy
  • regenerative medicine
  • retinal ganglion cell
  • transplantation

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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