Role of TGF-β Signaling in Coupling Bone Remodeling

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

TGF-β signaling plays a key role in the temporal and spatial regulation of bone remodeling. During osteoclast bone resorption, TGF-β is released from the bone matrix and activated. Active TGF-β recruits mesenchymal stem cells to the bone resorption pit through the SMAD signaling pathway. Mesenchymal stem cells undergo osteoblast differentiation and deposit new bone fi lling in the resorption pit and maintaining the structural integrity of the skeleton. Thus, TGF-β signaling plays a key role in the coupling process and disruptions to the TGF-β signaling pathway lead to loss of skeletal integrity. This chapter describes methods on how to quantitate bone matrix TGF-β and assess its role in mesenchymal stem cell migration both in vitro and in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages287-300
Number of pages14
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1344
ISSN (Print)1064-3745

Keywords

  • Immunohistochemistry co-staining
  • Mesenchymal stem cells
  • Migration
  • SMAD
  • TGF-β1
  • TRAP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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