Role of tfxE, but not tfxG, in trifolitoxin resistance

Alexandra J. Scupham, Yuemei Dong, Eric W. Triplett

Research output: Contribution to journalArticlepeer-review

Abstract

Eight genes, tfxABCDEFG and tfuA, confer production of trifolitoxin (TFX), a ribosomally synthesized, posttranslationally modified peptide antihiotic, in TFX-sensitive α-proteohacteria. An in-frame deletion in tfxE significantly reduced a strain's resistance to TFX in comparison to that of an otherwise identical construct containing wild-type tfxE. The deletion of tfxG had no effect on TFX resistance. Nevertheless, RNase protection assays showed that tfxE and tfxG are transcrihed, showing that the tfxDEFG mRNA was produced on the same transcript. Examination of the role of tfxG in TFX production showed that the tfxG mutant expressed slightly less TFX activity and produced only one TFX isomer while four are produced by the wild-type strain. Thus, tfxE plays an important role in TFX resistance while tfxG is important in optimal TFX production through the production of TFX isomers.

Original languageEnglish (US)
Pages (from-to)4334-4340
Number of pages7
JournalApplied and environmental microbiology
Volume68
Issue number9
DOIs
StatePublished - Sep 2002

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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