TY - JOUR
T1 - Role of tfxE, but not tfxG, in trifolitoxin resistance
AU - Scupham, Alexandra J.
AU - Dong, Yuemei
AU - Triplett, Eric W.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/9
Y1 - 2002/9
N2 - Eight genes, tfxABCDEFG and tfuA, confer production of trifolitoxin (TFX), a ribosomally synthesized, posttranslationally modified peptide antihiotic, in TFX-sensitive α-proteohacteria. An in-frame deletion in tfxE significantly reduced a strain's resistance to TFX in comparison to that of an otherwise identical construct containing wild-type tfxE. The deletion of tfxG had no effect on TFX resistance. Nevertheless, RNase protection assays showed that tfxE and tfxG are transcrihed, showing that the tfxDEFG mRNA was produced on the same transcript. Examination of the role of tfxG in TFX production showed that the tfxG mutant expressed slightly less TFX activity and produced only one TFX isomer while four are produced by the wild-type strain. Thus, tfxE plays an important role in TFX resistance while tfxG is important in optimal TFX production through the production of TFX isomers.
AB - Eight genes, tfxABCDEFG and tfuA, confer production of trifolitoxin (TFX), a ribosomally synthesized, posttranslationally modified peptide antihiotic, in TFX-sensitive α-proteohacteria. An in-frame deletion in tfxE significantly reduced a strain's resistance to TFX in comparison to that of an otherwise identical construct containing wild-type tfxE. The deletion of tfxG had no effect on TFX resistance. Nevertheless, RNase protection assays showed that tfxE and tfxG are transcrihed, showing that the tfxDEFG mRNA was produced on the same transcript. Examination of the role of tfxG in TFX production showed that the tfxG mutant expressed slightly less TFX activity and produced only one TFX isomer while four are produced by the wild-type strain. Thus, tfxE plays an important role in TFX resistance while tfxG is important in optimal TFX production through the production of TFX isomers.
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U2 - 10.1128/AEM.68.9.4334-4340.2002
DO - 10.1128/AEM.68.9.4334-4340.2002
M3 - Article
C2 - 12200284
AN - SCOPUS:0036731605
VL - 68
SP - 4334
EP - 4340
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 9
ER -