Role of testosterone binding to the androgen receptor in male sexual differentiation of patients with 5α-reductase deficiency

Marc Maes, Charles Sultan, Nadia Zerhouni, Stephen W. Rothwell, Claude J. Migeon

Research output: Contribution to journalArticle

Abstract

Testosterone (T) and dihydrotestosterone (DHT) were found to bind to a specific protein in genital skin fibroblasts of a patient with 5α-reductase deficiency. While maximum number of binding sites (Bmax) were similar for both androgens, the apparent dissociation constant (KD) of the androgen receptor for T was somewhat greater than for DHT. In competition studies of [3H]-T bound to the receptor with unlabeled T or DHT, the inhibitor constant (Ki) for T was two to three fold greater than the Ki for DHT. Also, the dissociation rate constant (kD) for [3H]-T bound to the receptor was greater than for [3H]-DHT (t 1 2 for T = 10 h and t 1 2 for DHT = 74.5 h). These results suggest that T may play a role in the sexual differentiation of male patients with 5α-reductase deficiency during their fetal life and at puberty. The low degree of masculinization during fetal life would be explained by the lower affinity and faster turnover rate of the T-reeeptor complex relative to the DHT-receptor complex. The relatively greater masculinization observed at puberty would be explained by the higher plasma T levels attained in the adult when compared to the fetus.

Original languageEnglish (US)
Pages (from-to)1385-1390
Number of pages6
JournalJournal of Steroid Biochemistry
Volume11
Issue number4
DOIs
StatePublished - 1979

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Sex Differentiation
Dihydrotestosterone
Androgen Receptors
Testosterone
Oxidoreductases
Puberty
Fibroblasts
Theophylline
Androgens
Rate constants
Skin
Fetus
Binding Sites
Plasmas
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Role of testosterone binding to the androgen receptor in male sexual differentiation of patients with 5α-reductase deficiency. / Maes, Marc; Sultan, Charles; Zerhouni, Nadia; Rothwell, Stephen W.; Migeon, Claude J.

In: Journal of Steroid Biochemistry, Vol. 11, No. 4, 1979, p. 1385-1390.

Research output: Contribution to journalArticle

Maes, Marc ; Sultan, Charles ; Zerhouni, Nadia ; Rothwell, Stephen W. ; Migeon, Claude J. / Role of testosterone binding to the androgen receptor in male sexual differentiation of patients with 5α-reductase deficiency. In: Journal of Steroid Biochemistry. 1979 ; Vol. 11, No. 4. pp. 1385-1390.
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