Abstract
The effects of oxidized human plasma low density lipoproteins (Ox-LDL) on the proliferation of cultured aortic smooth muscle cells was studied, employing viable cell counting, [3H] thymidine incorporation into DNA, and the release of lactate dehydrogenase (LDH) into the medium. Oxidized LDL (prepared by incubation of LDL with copper sulfate) exerted a concentration-dependent stimulation (2 fold, compared to control) of aortic smooth muscle cell proliferation at low concentrations (0.1 μg - 10 μg/ml medium). On the other hand, at high concentrations (25-200 μg/ml), Ox-LDL produced a pronounced decrease in viable cells, a decrease in the incorporation of [3H] thymidine into DNA, and an increase in the release of LDH in the medium. In this report, the previously postulated biological roles of oxidized-LDL in atherosclerosis are discussed in view of these findings.
Original language | English (US) |
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Pages (from-to) | 143-147 |
Number of pages | 5 |
Journal | Molecular and Cellular Biochemistry |
Volume | 111 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 1992 |
Keywords
- atherosclerosis
- oxidized low density lipoproteins
- smooth muscle cell proliferation
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology