Role of Na+-glucose cotransport in jejunal meal-induced absorption

Oscar J. Hines, Edward E. Whang, Anton J. Bilchik, Michael J. Zinner, Mark L. Welton, John Lane, David W. McFadden, Stanley W. Ashley

Research output: Contribution to journalArticle

Abstract

In awake dogs, meal ingestion stimulates the absorption of water and electrolytes from neurovascularly intact jejunal Thiry-Vella loops, even though these loops are isolated from the remainder of the gut. This study was designed to investigate the role of Na+-glucose cotransport in mediating this event. Meal ingestion enhanced absorption when the jejunal lumen was perfused with an isotonic solution containing D-glucose, D-galactose, or 3-O- methylglucose. This response was absent when the perfusate contained mannitol or when phlorizin was added to the D-glucose solution. Mucosa from the jejunal loops was serially biopsied and assayed for brush-border Na+-glucose cotransporter (SGLT1) mRNA and protein expression. Although no changes in SGLT1 mRNA levels were observed, protein levels significantly increased within 30 min following meal ingestion. The time course of SGLT1 protein expression corresponded with that of increased Na+ and water absorption. These results suggest that meal-stimulated jejunal absorption may be mediated through an induction of mucosal SGLT1.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalDigestive Diseases and Sciences
Volume45
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Meals
Sodium-Glucose Transporter 1
Glucose
Eating
3-O-Methylglucose
Phlorhizin
Isotonic Solutions
Messenger RNA
Water
Mannitol
Microvilli
Galactose
Electrolytes
Mucous Membrane
Dogs
Proteins

Keywords

  • Intestinal absorption
  • Intestinal adaptation
  • SGLT1
  • Thiry-Vella loop

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Hines, O. J., Whang, E. E., Bilchik, A. J., Zinner, M. J., Welton, M. L., Lane, J., ... Ashley, S. W. (2000). Role of Na+-glucose cotransport in jejunal meal-induced absorption. Digestive Diseases and Sciences, 45(1), 1-6. https://doi.org/10.1023/A:1005477204960

Role of Na+-glucose cotransport in jejunal meal-induced absorption. / Hines, Oscar J.; Whang, Edward E.; Bilchik, Anton J.; Zinner, Michael J.; Welton, Mark L.; Lane, John; McFadden, David W.; Ashley, Stanley W.

In: Digestive Diseases and Sciences, Vol. 45, No. 1, 2000, p. 1-6.

Research output: Contribution to journalArticle

Hines, OJ, Whang, EE, Bilchik, AJ, Zinner, MJ, Welton, ML, Lane, J, McFadden, DW & Ashley, SW 2000, 'Role of Na+-glucose cotransport in jejunal meal-induced absorption', Digestive Diseases and Sciences, vol. 45, no. 1, pp. 1-6. https://doi.org/10.1023/A:1005477204960
Hines, Oscar J. ; Whang, Edward E. ; Bilchik, Anton J. ; Zinner, Michael J. ; Welton, Mark L. ; Lane, John ; McFadden, David W. ; Ashley, Stanley W. / Role of Na+-glucose cotransport in jejunal meal-induced absorption. In: Digestive Diseases and Sciences. 2000 ; Vol. 45, No. 1. pp. 1-6.
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