Role of MEF feeder cells in direct reprogramming of mousetail-tip fibroblasts

Mengfei Chen; Xuerong Sun; Ruzhang Jiang; Wenjuan Shen; Xiufeng Zhong; Bingqian Liu; Ying Qi; Bing Huang; Andy Peng Xiang; Jian Ge

doi:10.1016/j.cellbi.2009.06.004

Role of MEF feeder cells in direct reprogramming of mousetail-tip fibroblasts

Mengfei Chen, Xuerong Sun, Ruzhang Jiang, Wenjuan Shen, Xiufeng Zhong, Bingqian Liu, Ying Qi, Bing Huang, Andy Peng Xiang, Jian Ge

School of Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Pluripotent stem cells can be induced from somatic cells by the transcription factors Oct3/4, Sox2, c-Myc and Klf4 when co-cultured with mouse embryonic fibroblast (MEF) feeder cells. To date, the role of the feeder cells in the reprogramming process remains unclear. In this study, using a comparative analysis, we demonstrated that MEF feeder cells did not accelerate reprogramming or increase the frequency of induced pluripotent stem (iPS) cell colonies. However, feeder conditions did improve the growth of primary iPS colonies and were necessary for passaging the primary colonies after reprogramming was achieved. We further developed a feeder-free culture system for supporting iPS growth and sustaining pluripotency by adding bFGF and activin A (bFA) to the medium. These data will facilitate the generation of human iPS cells without animal feeders for regenerative medicine. Crown

Original language	English (US)
Pages (from-to)	1268-1273
Number of pages	6
Journal	Cell biology international
Volume	33
Issue number	12
DOIs	https://doi.org/10.1016/j.cellbi.2009.06.004
State	Published - Dec 2009

Keywords

Embryonic stem cells
Feeder-free
Induced pluripotent stem cells
MEF feeder cells
Reprogramming
fibroblasts

ASJC Scopus subject areas

Cell Biology

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10.1016/j.cellbi.2009.06.004

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title = "Role of MEF feeder cells in direct reprogramming of mousetail-tip fibroblasts",

abstract = "Pluripotent stem cells can be induced from somatic cells by the transcription factors Oct3/4, Sox2, c-Myc and Klf4 when co-cultured with mouse embryonic fibroblast (MEF) feeder cells. To date, the role of the feeder cells in the reprogramming process remains unclear. In this study, using a comparative analysis, we demonstrated that MEF feeder cells did not accelerate reprogramming or increase the frequency of induced pluripotent stem (iPS) cell colonies. However, feeder conditions did improve the growth of primary iPS colonies and were necessary for passaging the primary colonies after reprogramming was achieved. We further developed a feeder-free culture system for supporting iPS growth and sustaining pluripotency by adding bFGF and activin A (bFA) to the medium. These data will facilitate the generation of human iPS cells without animal feeders for regenerative medicine. Crown",

keywords = "Embryonic stem cells, Feeder-free, Induced pluripotent stem cells, MEF feeder cells, Reprogramming, fibroblasts",

author = "Mengfei Chen and Xuerong Sun and Ruzhang Jiang and Wenjuan Shen and Xiufeng Zhong and Bingqian Liu and Ying Qi and Bing Huang and Xiang, {Andy Peng} and Jian Ge",

note = "Funding Information: We would like to thank Drs Duan Shan and Gao Qianying for critical suggestions, and Dr Jing Zhuang for critical reading of this manuscript. This study was supported by the National Basic Research Program of China (973 program) NO: 2007CB512207 and the National Natural Science Foundation of China NO: 30672275, NO: 30500555 and NO: 30600695.",

year = "2009",

month = dec,

doi = "10.1016/j.cellbi.2009.06.004",

language = "English (US)",

volume = "33",

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journal = "Cell biology international",

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AU - Chen, Mengfei

AU - Sun, Xuerong

AU - Jiang, Ruzhang

AU - Shen, Wenjuan

AU - Zhong, Xiufeng

AU - Liu, Bingqian

AU - Qi, Ying

AU - Huang, Bing

AU - Xiang, Andy Peng

AU - Ge, Jian

N1 - Funding Information: We would like to thank Drs Duan Shan and Gao Qianying for critical suggestions, and Dr Jing Zhuang for critical reading of this manuscript. This study was supported by the National Basic Research Program of China (973 program) NO: 2007CB512207 and the National Natural Science Foundation of China NO: 30672275, NO: 30500555 and NO: 30600695.

PY - 2009/12

Y1 - 2009/12

N2 - Pluripotent stem cells can be induced from somatic cells by the transcription factors Oct3/4, Sox2, c-Myc and Klf4 when co-cultured with mouse embryonic fibroblast (MEF) feeder cells. To date, the role of the feeder cells in the reprogramming process remains unclear. In this study, using a comparative analysis, we demonstrated that MEF feeder cells did not accelerate reprogramming or increase the frequency of induced pluripotent stem (iPS) cell colonies. However, feeder conditions did improve the growth of primary iPS colonies and were necessary for passaging the primary colonies after reprogramming was achieved. We further developed a feeder-free culture system for supporting iPS growth and sustaining pluripotency by adding bFGF and activin A (bFA) to the medium. These data will facilitate the generation of human iPS cells without animal feeders for regenerative medicine. Crown

AB - Pluripotent stem cells can be induced from somatic cells by the transcription factors Oct3/4, Sox2, c-Myc and Klf4 when co-cultured with mouse embryonic fibroblast (MEF) feeder cells. To date, the role of the feeder cells in the reprogramming process remains unclear. In this study, using a comparative analysis, we demonstrated that MEF feeder cells did not accelerate reprogramming or increase the frequency of induced pluripotent stem (iPS) cell colonies. However, feeder conditions did improve the growth of primary iPS colonies and were necessary for passaging the primary colonies after reprogramming was achieved. We further developed a feeder-free culture system for supporting iPS growth and sustaining pluripotency by adding bFGF and activin A (bFA) to the medium. These data will facilitate the generation of human iPS cells without animal feeders for regenerative medicine. Crown

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Role of MEF feeder cells in direct reprogramming of mousetail-tip fibroblasts

Abstract

Keywords

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