Role of lipid rafts in Shiga toxin 1 interaction with the apical surface of Caco-2 cells

Research output: Contribution to journalArticle

Abstract

Enterohemorrhagic Escherichia coli producing Shiga toxins 1 and/or 2 have become major foodborne pathogens. The specific binding of Shiga toxin 1 B-subunit to its receptor, a neutral glycolipid globotriaosylceramide Gb3, on the apical surface of colonic epithelium followed by toxin entry into cells are the initial steps of the process, which can result in toxin transcytosis and systemic effects of infection including hemolytic uremic syndrome. Understanding the complex mechanisms of Shiga toxin 1 binding and internalization may help to develop new strategies directed at preventing toxin internalization. Fluorescence resonance energy transfer microscopy revealed the clustering of Shiga toxin receptors Gb3 in lipid rafts with another glycosphingolipid GM1 on the apical surface of highly polarized intestinal epithelial Caco-2 cells. Lipid rafts disruption significantly decreased internalization of Shiga toxin 1 B-subunit. Although disruption of lipid rafts by cholesterol depletion did not affect the amount of bound Shiga toxin 1 B-subunit, lipid rafts are necessary for toxin uptake across the apical membrane of Caco-2 cells.

Original languageEnglish (US)
Pages (from-to)4025-4031
Number of pages7
JournalJournal of cell science
Volume114
Issue number22
StatePublished - Dec 17 2001

Keywords

  • Caco-2 cells
  • Cholesterol
  • Globotriaosylceramide Gb
  • Lipid rafts
  • Shiga toxin

ASJC Scopus subject areas

  • Cell Biology

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