Role of inter-α-inhibitor and its related proteins in experimentally induced calcium oxalate urolithiasis. Localization of proteins and expression of bikunin gene in the rat kidney

Fouad Atmani, Patricia A. Glenton, Saeed R. Khan

Research output: Contribution to journalArticle

Abstract

Our earlier studies indicated that members of the inter-α-inhibitor (IαI) family of glycoproteins may play an important role in urolithiasis. Indeed bikunin, the light chain of IαI is a potent inhibitor of calcium oxalate crystallization. In order to understand this role, the distribution of IαI and its related proteins, as well as the expression of bikunin, were studied in normal and nephrolithic rats. In normal rats, IαI immunoreactivity was located mainly in proximal tubules. However, in nephrolithic rats, in addition to proximal tubules, the staining was intensively extended to tubules in the corricomedullary junction. Furthermore, by using polymerase chain reaction technique, we demonstrated that gene encoding for bikunin was activated in kidneys of nephrolithic rats. We have previously demonstrated increased staining for osteopontin in association with calcium oxalate crystal deposition in rat kidneys. Others have shown an increase in osteopontin production by renal epithelial cells on exposure to calcium oxalate crystals. Based on these observations we conclude that kidney cells possess an auto-defense system against calcium oxalate crystallization and stone formation in which members of the IαI family may be closely involved.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalUrological Research
Volume27
Issue number1
DOIs
StatePublished - Feb 1999
Externally publishedYes

Fingerprint

Calcium Oxalate
Kidney
Gene Expression
Osteopontin
Crystallization
Proteins
Staining and Labeling
Urolithiasis
Glycoproteins
Epithelial Cells
Calcium Oxalate Nephrolithiasis
Light
Polymerase Chain Reaction
Genes

Keywords

  • Bikunin
  • Calcium oxalate crystallization
  • Interα- inhibitor
  • Kidneys
  • Nephrolithiasis

ASJC Scopus subject areas

  • Urology

Cite this

Role of inter-α-inhibitor and its related proteins in experimentally induced calcium oxalate urolithiasis. Localization of proteins and expression of bikunin gene in the rat kidney. / Atmani, Fouad; Glenton, Patricia A.; Khan, Saeed R.

In: Urological Research, Vol. 27, No. 1, 02.1999, p. 63-67.

Research output: Contribution to journalArticle

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