TY - JOUR
T1 - Role of HuR in skeletal myogenesis through coordinate regulation of muscle differentiation genes
AU - Figueroa, Angélica
AU - Cuadrado, Ana
AU - Fan, Jinshui
AU - Atasoy, Ulus
AU - Muscat, George E.
AU - Muñoz-Canoves, Pura
AU - Gorospe, Myriam
AU - Muñoz, Alberto
PY - 2003/7
Y1 - 2003/7
N2 - In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse muscle, HuR cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion of myogenesis. mRNAs encoding key regulators of myogenesis-specific transcription (myogenin and MyoD) and cell cycle withdrawal (p21), bearing AU-rich regions, were found to be targets of HuR in a differentiation-dependent manner. Accordingly, mRNA half-lives were highest during differentiation, declining when differentiation was completed. Importantly, HuR-overexpressing C2C12 cells displayed increased target mRNA expression and half-life and underwent precocious differentiation. Our findings underscore a critical function for HuR during skeletal myogenesis linked to HuR's coordinate regulation of muscle differentiation genes.
AB - In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse muscle, HuR cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion of myogenesis. mRNAs encoding key regulators of myogenesis-specific transcription (myogenin and MyoD) and cell cycle withdrawal (p21), bearing AU-rich regions, were found to be targets of HuR in a differentiation-dependent manner. Accordingly, mRNA half-lives were highest during differentiation, declining when differentiation was completed. Importantly, HuR-overexpressing C2C12 cells displayed increased target mRNA expression and half-life and underwent precocious differentiation. Our findings underscore a critical function for HuR during skeletal myogenesis linked to HuR's coordinate regulation of muscle differentiation genes.
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U2 - 10.1128/MCB.23.14.4991-5004.2003
DO - 10.1128/MCB.23.14.4991-5004.2003
M3 - Article
C2 - 12832484
AN - SCOPUS:0038450334
SN - 0270-7306
VL - 23
SP - 4991
EP - 5004
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 14
ER -