Role of HuR in skeletal myogenesis through coordinate regulation of muscle differentiation genes

Angélica Figueroa, Ana Cuadrado, Jinshui Fan, Ulus Atasoy, George E. Muscat, Pura Muñoz-Canoves, Myriam Gorospe, Alberto Muñoz

Research output: Contribution to journalArticlepeer-review

Abstract

In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse muscle, HuR cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion of myogenesis. mRNAs encoding key regulators of myogenesis-specific transcription (myogenin and MyoD) and cell cycle withdrawal (p21), bearing AU-rich regions, were found to be targets of HuR in a differentiation-dependent manner. Accordingly, mRNA half-lives were highest during differentiation, declining when differentiation was completed. Importantly, HuR-overexpressing C2C12 cells displayed increased target mRNA expression and half-life and underwent precocious differentiation. Our findings underscore a critical function for HuR during skeletal myogenesis linked to HuR's coordinate regulation of muscle differentiation genes.

Original languageEnglish (US)
Pages (from-to)4991-5004
Number of pages14
JournalMolecular and cellular biology
Volume23
Issue number14
DOIs
StatePublished - Jul 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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