Role of genitourinary inflammation in infertility: Synergistic effect of lipopolysaccharide and interferon-γ on human spermatozoa

Suresh C. Sikka, Hunter C. Champion, Trinity Bivalacqua, Lance S. Estrada, Run Wang, Mahadevan Rajasekaran, Bharat B. Aggarwal, Wayne J G Hellstrom

Research output: Contribution to journalArticle

Abstract

Pro-inflammatory cytokines are elevated in the semen of patients with genitourinary inflammation (GUI). Whether this increase in cytokines in GUI patients plays any critical role in male factor infertility is not clear. The present study investigated the in vitro effects of two important pro-inflammatory cytokines, lipopolysaccharide (LPS) and interferon-γ (IFN-γ), on sperm motility, viability, membrane integrity and motion parameters. Washed spermatozoa from healthy donors were incubated with LPS (0.1 mg/mL) or IFN-γ (0.1 mg/mL) alone or in combination. Sperm motility, viability, membrane integrity and computer-assisted motion were evaluated at various time intervals (0, 30, 60 and 180 min) after treatment. Sperm membrane integrity was analysed using the hypoosmotic swelling test (HOST). LPS and IFN-γ individually did not alter sperm viability or motility, but their combination showed a significant time-dependent decrease (p <0.05) in sperm motility, viability and membrane integrity. Sperm motion parameters (straight-line velocity, curvilinear velocity, mean linearity, or amplitude of lateral head displacement) were not affected by LPS or IFN-γ at the concentrations used in this study. These data suggest that the combination of LPS and IFN-γ is detrimental to human spermatozoa and may contribute to male factor infertility in patients with chronic GUI.

Original languageEnglish (US)
Pages (from-to)136-141
Number of pages6
JournalInternational Journal of Andrology
Volume24
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Fertility Agents
Interferons
Lipopolysaccharides
Spermatozoa
Sperm Motility
Inflammation
Membranes
Male Infertility
Cytokines
Semen
Tissue Donors

Keywords

  • Genitourinary inflammation
  • Human sperm motion/viability
  • IFN-γ
  • Infertility
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Endocrinology

Cite this

Role of genitourinary inflammation in infertility : Synergistic effect of lipopolysaccharide and interferon-γ on human spermatozoa. / Sikka, Suresh C.; Champion, Hunter C.; Bivalacqua, Trinity; Estrada, Lance S.; Wang, Run; Rajasekaran, Mahadevan; Aggarwal, Bharat B.; Hellstrom, Wayne J G.

In: International Journal of Andrology, Vol. 24, No. 3, 2001, p. 136-141.

Research output: Contribution to journalArticle

Sikka, Suresh C. ; Champion, Hunter C. ; Bivalacqua, Trinity ; Estrada, Lance S. ; Wang, Run ; Rajasekaran, Mahadevan ; Aggarwal, Bharat B. ; Hellstrom, Wayne J G. / Role of genitourinary inflammation in infertility : Synergistic effect of lipopolysaccharide and interferon-γ on human spermatozoa. In: International Journal of Andrology. 2001 ; Vol. 24, No. 3. pp. 136-141.
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