Role of endogenous glucagon-like peptide-1 in islet regeneration after partial pancreatectomy

Diva D. De León, Shaoping Deng, Reza Madani, Rexford S. Ahima, Daniel J. Drucker, Doris A. Stoffers

Research output: Contribution to journalArticlepeer-review

Abstract

A reduction in β-cell mass is an important causative factor in type 1 and type 2 diabetes. Glucagon-like peptide-1 (GLP-1) and the long-acting agonist exendin 4 (Ex-4) expand β-cell mass by stimulating neogenesis and proliferation. In the partial pancreatectomy (Ppx) model, exogenous Ex-4 promotes islet regeneration, leading to sustained improvement in glucose tolerance. In this study, we investigate the potential role of endogenous GLP-1 in islet growth. We examined β-cell mass regeneration after 70% Ppx in mice receiving the GLP-1 antagonist Ex9-39 and in GLP-1R-/- mice. In Ex9-39-treated sham-operated mice, persistent fasting hyperglycemia was observed, but β-cell mass was not diminished. In pancreatectomized mice, persistent glucose intolerance was noted, but this was not further exacerbated by Ex9-39. Accordingly, β-cell mass recovery of Ppx mice was not impaired by Ex9-39. In contrast, GLP-1R-/- CD1 mice showed worse glucose intolerance after Ppx compared with wild-type CD1 Ppx mice, and this correlated with a significant defect in β-cell mass regeneration. The recovery of β-cell mass differed markedly in the BALB/c and CD1 control mice, indicating a significant role of genetic background in the regulation of β-cell mass. These studies point to a role for endogenous GLP-1 in β-cell regeneration after Ppx in mice.

Original languageEnglish (US)
Pages (from-to)365-371
Number of pages7
JournalDiabetes
Volume52
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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