Role of cyanobacteria in the persistence of Vibrio cholerae O139 in saline microcosms

M. S. Islam, S. Mahmuda, M. G. Morshed, H. B.M. Bakht, M. N.H. Khan, R. B. Sack, D. A. Sack

Research output: Contribution to journalArticle

Abstract

Recently, a new strain of cholera, Vibrio cholerae O139, has emerged as an epidemic strain, but there is little information about its environmental reservoir. The present investigation was aimed to determine the role of cyanobacteria in the persistence of V. cholerae O139 in microcosms. An environmental isolate of V. cholerae O139 and three cyanobacteria (Anabaena sp., Nostoc sp., and Hapalosiphon sp.) were used in this study. Survival of culturable V. cholerae O139 in microcosms was monitored using taurocholate-tellurite gelatin agar medium. Viable but nonculturable V. cholerae O139 were detected using a fluorescent antibody technique. Vibrio cholerae O139 could be isolated for up to 12 days in a culturable form in association with cyanobacteria but could not be isolated in the culturable form after 2 days from control water without cyanobacteria. The viable but nonculturable V. cholerae O139 could be detected in association with cyanobacteria for up to 15 months. These results, therefore, suggest that cyanobacteria can act as a long-term reservoir of V. cholerae O139 in an aquatic environment.

Original languageEnglish (US)
Pages (from-to)127-131
Number of pages5
JournalCanadian Journal of Microbiology
Volume50
Issue number2
DOIs
StatePublished - Feb 1 2004

Keywords

  • Cyanobacteria
  • Microcosm
  • Persistence
  • Reservoir
  • Vibrio cholerae

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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    Islam, M. S., Mahmuda, S., Morshed, M. G., Bakht, H. B. M., Khan, M. N. H., Sack, R. B., & Sack, D. A. (2004). Role of cyanobacteria in the persistence of Vibrio cholerae O139 in saline microcosms. Canadian Journal of Microbiology, 50(2), 127-131. https://doi.org/10.1139/w03-114