Role of complement regulatory proteins in the survival of murine allo-transplanted Sertoli cells

Hak Mo Lee, Byoung Chol Oh, Dong Pyo Lim, Dong Sup Lee, Jaejin Cho, Gene Lee, Ryul Lee Jeong

Research output: Contribution to journalArticle

Abstract

Sertoli cells (SC) are known to contain immunoprotective properties, which allow them to survive as allografts without the use of immunosuppressive drugs. Experiments were designed to determine which factors are related to prolonged survival of allogeneic SC. Balb/c derived Sertoli (TM4) and colon cancer (CT-26) cell lines were implanted beneath the kidney capsule of non-immunosuppressed C57BL/6 mice and compared their survival as allografts. Compared to TM4 graft, which survived more than 7 days after transplantation, CT-26 showed massive infiltration of polymorphonuclear cells, necrosis and enlargement of draining lymph nodes. Cultured cell lines showed no differences in their expression patterns of FasL, TGF 1, clusterin and two complement regulatory proteins (CRP, i.e., membrane cofactor protein, MCP; decay accelerating factor, DAF), but protectin (CD59), another member of CRP was expressed only on TM4. These results suggest that CD59 and unknown factors may contribute to the prolonged survival of SC in non-immunoprivileged sites.

Original languageEnglish (US)
Pages (from-to)277-282
Number of pages6
JournalJournal of Korean Medical Science
Volume22
Issue number2
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

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Sertoli Cells
Complement System Proteins
Allografts
CD59 Antigens
CD46 Antigens
CD55 Antigens
Cell Enlargement
Clusterin
Cell Line
Immunosuppressive Agents
Inbred C57BL Mouse
Colonic Neoplasms
Capsules
Cultured Cells
Necrosis
Transplantation
Lymph Nodes
Transplants
Kidney
Pharmaceutical Preparations

Keywords

  • CD59
  • Clusterin
  • Complement regulatory protein
  • Fas ligand
  • Sertoli cells
  • Transfroming growth factor beta1

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Role of complement regulatory proteins in the survival of murine allo-transplanted Sertoli cells. / Lee, Hak Mo; Oh, Byoung Chol; Lim, Dong Pyo; Lee, Dong Sup; Cho, Jaejin; Lee, Gene; Jeong, Ryul Lee.

In: Journal of Korean Medical Science, Vol. 22, No. 2, 01.01.2007, p. 277-282.

Research output: Contribution to journalArticle

Lee, Hak Mo ; Oh, Byoung Chol ; Lim, Dong Pyo ; Lee, Dong Sup ; Cho, Jaejin ; Lee, Gene ; Jeong, Ryul Lee. / Role of complement regulatory proteins in the survival of murine allo-transplanted Sertoli cells. In: Journal of Korean Medical Science. 2007 ; Vol. 22, No. 2. pp. 277-282.
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