Role of chromatographic techniques in proteomic analysis

Irina Neverova, Jennifer E. Van Eyk

Research output: Contribution to journalArticle

Abstract

Proteomics, the characterization of the proteome, is conceptually simple but technically challenging. Development of such technologies as mass spectrometry, multidimensional protein separation, and DNA sequencing has allowed the new field of proteomics to flourish. Proteomic analysis relies on a set of techniques chosen on the basis of the biological question. In any proteomic analysis, the first and most important task is the separation of a complex protein mixture, i.e. the proteome. Chromatography, one of the most powerful methods of separation, employs one or more inherent characteristics of a protein - its mass, isoelectric point, hydrophobicity or biospecificity. This review emphasizes high-performance liquid chromatography as an integrated part of technologies used to study the proteome, discusses the capabilities and limitations of current instruments, and highlights the potential of multidimensional liquid chromatography in proteomic analysis.

Original languageEnglish (US)
Pages (from-to)51-63
Number of pages13
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume815
Issue number1-2
DOIs
Publication statusPublished - Feb 5 2005

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Keywords

  • Liquid chromatography-mass spectrometry
  • Multidimensional approach
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry

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