TY - JOUR
T1 - Role of chromatographic techniques in proteomic analysis
AU - Neverova, Irina
AU - Van Eyk, Jennifer E.
N1 - Funding Information:
J.E. Van Eyk is funded by the Canadian Institutes of Health Research, the Ontario Heart and Stroke Foundation, and the National Institutes of Health (USA).
PY - 2005/2/5
Y1 - 2005/2/5
N2 - Proteomics, the characterization of the proteome, is conceptually simple but technically challenging. Development of such technologies as mass spectrometry, multidimensional protein separation, and DNA sequencing has allowed the new field of proteomics to flourish. Proteomic analysis relies on a set of techniques chosen on the basis of the biological question. In any proteomic analysis, the first and most important task is the separation of a complex protein mixture, i.e. the proteome. Chromatography, one of the most powerful methods of separation, employs one or more inherent characteristics of a protein - its mass, isoelectric point, hydrophobicity or biospecificity. This review emphasizes high-performance liquid chromatography as an integrated part of technologies used to study the proteome, discusses the capabilities and limitations of current instruments, and highlights the potential of multidimensional liquid chromatography in proteomic analysis.
AB - Proteomics, the characterization of the proteome, is conceptually simple but technically challenging. Development of such technologies as mass spectrometry, multidimensional protein separation, and DNA sequencing has allowed the new field of proteomics to flourish. Proteomic analysis relies on a set of techniques chosen on the basis of the biological question. In any proteomic analysis, the first and most important task is the separation of a complex protein mixture, i.e. the proteome. Chromatography, one of the most powerful methods of separation, employs one or more inherent characteristics of a protein - its mass, isoelectric point, hydrophobicity or biospecificity. This review emphasizes high-performance liquid chromatography as an integrated part of technologies used to study the proteome, discusses the capabilities and limitations of current instruments, and highlights the potential of multidimensional liquid chromatography in proteomic analysis.
KW - Liquid chromatography-mass spectrometry
KW - Multidimensional approach
KW - Proteomics
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U2 - 10.1016/j.jchromb.2004.11.009
DO - 10.1016/j.jchromb.2004.11.009
M3 - Review article
C2 - 15652798
AN - SCOPUS:12344330739
SN - 1570-0232
VL - 815
SP - 51
EP - 63
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1-2
ER -