Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts

Takuma Fukunishi, Cameron A. Best, Chin Siang Ong, Tyler Groehl, James Reinhardt, Tai Yi, Hideki Miyachi, Huaitao Zhang, Toshiharu Shinoka, Christopher K. Breuer, Jed Johnson, Narutoshi Hibino

Research output: Contribution to journalArticle

Abstract

Objective: Electrospinning is a promising technology that provides biodegradable nanofiber scaffolds for cardiovascular tissue engineering. However, success with these materials has been limited, and the optimal combination of scaffold parameters for a tissue-engineered vascular graft (TEVG) remains elusive. The purpose of the present study is to evaluate the effect of bone marrow mononuclear cell (BM-MNC) seeding in electrospun scaffolds to support the rational design of optimized TEVGs. Methods: Nanofiber scaffolds were fabricated from co-electrospinning a solution of polyglycolic acid and a solution of poly(ι-lactide-co-ϵ-caprolactone) and characterized with scanning electron microscopy. Platelet activation and cell seeding efficiency were assessed by ATP secretion and DNA assays, respectively. Cell-free and BM-MNC seeded scaffolds were implanted in C57BL/6 mice (n = 15/group) as infrarenal inferior vena cava (IVC) interposition conduits. Animals were followed with serial ultrasonography for 6 months, after which grafts were harvested for evaluation of patency and neotissue formation by histology and immunohistochemistry (n = 10/group) and PCR (n = 5/group) analyses. Results: BM-MNC seeding of electrospun scaffolds prevented stenosis compared with unseeded scaffolds (seeded: 9/10 patent vs. unseeded: 1/10 patent, p = 0.0003). Seeded vascular grafts demonstrated concentric laminated smooth muscle cells, a confluent endothelial monolayer, and a collagen-rich extracellular matrix. Platelet-derived ATP, a marker of platelet activation, was significantly reduced after incubating thrombin-activated platelets in the presence of seeded scaffolds compared with unseeded scaffolds (p < 0.0001). In addition, reduced macrophage infiltration and a higher M2 macrophage percentage were observed in seeded grafts. Conclusions: The beneficial effects of BM-MNC seeding apply to electrospun TEVG scaffolds by attenuating stenosis through the regulation of platelet activation and inflammatory macrophage function, leading to well-organized neotissue formation. BM-MNC seeding is a valuable technique that can be used in the rational design of optimal TEVG scaffolds.

Original languageEnglish (US)
Pages (from-to)135-144
Number of pages10
JournalTissue Engineering - Part A
Volume24
Issue number1-2
DOIs
StatePublished - Jan 1 2018

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Nanofibers
Grafts
Scaffolds
Bone Marrow Cells
Blood Vessels
Bone
Blood Vessel Prosthesis
Transplants
Platelet Activation
Platelets
Macrophages
Pathologic Constriction
Blood Platelets
Adenosine Triphosphate
Polyglycolic Acid
Adenosinetriphosphate
Chemical activation
Electrospinning
Inferior Vena Cava
Tissue Engineering

Keywords

  • biodegradable scaffold
  • bone marrow mononuclear cell (BM-MNC) seeding
  • electrospinning
  • nanofiber
  • stenosis
  • Tissue-engineered vascular graft (TEVG)

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Fukunishi, T., Best, C. A., Ong, C. S., Groehl, T., Reinhardt, J., Yi, T., ... Hibino, N. (2018). Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts. Tissue Engineering - Part A, 24(1-2), 135-144. https://doi.org/10.1089/ten.tea.2017.0044

Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts. / Fukunishi, Takuma; Best, Cameron A.; Ong, Chin Siang; Groehl, Tyler; Reinhardt, James; Yi, Tai; Miyachi, Hideki; Zhang, Huaitao; Shinoka, Toshiharu; Breuer, Christopher K.; Johnson, Jed; Hibino, Narutoshi.

In: Tissue Engineering - Part A, Vol. 24, No. 1-2, 01.01.2018, p. 135-144.

Research output: Contribution to journalArticle

Fukunishi, T, Best, CA, Ong, CS, Groehl, T, Reinhardt, J, Yi, T, Miyachi, H, Zhang, H, Shinoka, T, Breuer, CK, Johnson, J & Hibino, N 2018, 'Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts', Tissue Engineering - Part A, vol. 24, no. 1-2, pp. 135-144. https://doi.org/10.1089/ten.tea.2017.0044
Fukunishi T, Best CA, Ong CS, Groehl T, Reinhardt J, Yi T et al. Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts. Tissue Engineering - Part A. 2018 Jan 1;24(1-2):135-144. https://doi.org/10.1089/ten.tea.2017.0044
Fukunishi, Takuma ; Best, Cameron A. ; Ong, Chin Siang ; Groehl, Tyler ; Reinhardt, James ; Yi, Tai ; Miyachi, Hideki ; Zhang, Huaitao ; Shinoka, Toshiharu ; Breuer, Christopher K. ; Johnson, Jed ; Hibino, Narutoshi. / Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts. In: Tissue Engineering - Part A. 2018 ; Vol. 24, No. 1-2. pp. 135-144.
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AU - Yi, Tai

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