Role of arginase in killing of schistosomula of Schistosoma mansoni

G. R. Olds, J. J. Ellner, L. A. Kearse, J. W. Kazura, A. A. Mahmoud

Research output: Contribution to journalArticle

Abstract

Nonspecific resistance to the multicellular organism Schistosoma mansoni can be induced in mice by several infectious agents. We utilized the observed genetic restriction of such acquired resistance to study the mediators of killing of the larval stage of S. mansoni in vitro. Adherent peritoneal cell monolayers from Corynebacterium parvum-treated C57BL/6J but not from C. parvum-treated BALB/cJ mice killed an increased proportion of schistosomula in 24 h. Activated macrophages (M∅) from both strains exhibited enhanced H 2O 2 production after incubation with the parasites or phorbol myristate acetate. Thus H 2O 2 production was not associated with schistosomula killing. Moreover, schistosomula killing was unaffected by catalase or superoxide dismutase. In contrast, activated C57BL/6J (but not BALB/cJ) M∅ released fourfold more arginase into supernates than control M∅. Schistosomula killing by these M∅ correlated with arginase content of the supernates, was exaggerated in arginine-poor medium, and could be blocked by the addition of arginine. Exogenous bovine arginase added to Fischer's medium without macrophages produced comparable parasite mortality. Our data suggest that arginase is a critical mediator of in vitro killing of this multicellular organism by activated macrophages.

Original languageEnglish (US)
Pages (from-to)1557-1562
Number of pages6
JournalJournal of Experimental Medicine
Volume151
Issue number6
StatePublished - 1980
Externally publishedYes

Fingerprint

Arginase
Schistosoma mansoni
Propionibacterium acnes
Macrophages
Arginine
Parasites
Tetradecanoylphorbol Acetate
Catalase
Superoxide Dismutase
Mortality
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Olds, G. R., Ellner, J. J., Kearse, L. A., Kazura, J. W., & Mahmoud, A. A. (1980). Role of arginase in killing of schistosomula of Schistosoma mansoni. Journal of Experimental Medicine, 151(6), 1557-1562.

Role of arginase in killing of schistosomula of Schistosoma mansoni. / Olds, G. R.; Ellner, J. J.; Kearse, L. A.; Kazura, J. W.; Mahmoud, A. A.

In: Journal of Experimental Medicine, Vol. 151, No. 6, 1980, p. 1557-1562.

Research output: Contribution to journalArticle

Olds, GR, Ellner, JJ, Kearse, LA, Kazura, JW & Mahmoud, AA 1980, 'Role of arginase in killing of schistosomula of Schistosoma mansoni', Journal of Experimental Medicine, vol. 151, no. 6, pp. 1557-1562.
Olds GR, Ellner JJ, Kearse LA, Kazura JW, Mahmoud AA. Role of arginase in killing of schistosomula of Schistosoma mansoni. Journal of Experimental Medicine. 1980;151(6):1557-1562.
Olds, G. R. ; Ellner, J. J. ; Kearse, L. A. ; Kazura, J. W. ; Mahmoud, A. A. / Role of arginase in killing of schistosomula of Schistosoma mansoni. In: Journal of Experimental Medicine. 1980 ; Vol. 151, No. 6. pp. 1557-1562.
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