Rod sensitivity of neonatal mouse and rat

Dong Gen Luo, King Wai Yau

Research output: Contribution to journalArticlepeer-review

Abstract

We have measured the sensitivity of rod photoreceptors isolated from overnight dark-adapted mice of age P12 (neonate) through P45 (adult) with suction-pipette recording. During this age period, the dark current increased roughly in direct proportion to the length of the rod outer segment. In the same period, the flash sensitivity of rods (reciprocal of the half-saturating flash intensity) increased by ∼ 1.5-fold. This slight developmental change in sensitivity was not accentuated by dark adapting the animal for just 1 h or by increasing the ambient luminance by sixfold during the prior light exposure. The same small, age-dependent change in rod sensitivity was found with rat. After preincubation of the isolated retina with 9-cis-retinal, neonatal mouse rods showed the same sensitivity as adult rods, suggesting the presence of a small amount of free opsin being responsible for their lower sensitivity. The sensitivity of neonate rods could also be increased to the adult level by dark adapting the animal continuously for several days. By comparing the sensitivity of neonate rods in darkness to that of adult rods after light bleaches, we estimated that ∼ 1% of rod opsin in neonatal mouse was devoid of chromophore even after overnight dark adaptation. Overall, we were unable to confirm a previous report that a 50-fold difference in rod sensitivity existed between neonatal and adult rats.

Original languageEnglish (US)
Pages (from-to)263-269
Number of pages7
JournalJournal of General Physiology
Volume126
Issue number3
DOIs
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Rod sensitivity of neonatal mouse and rat'. Together they form a unique fingerprint.

Cite this