Robust reprogramming of Ataxia-Telangiectasia patient and carrier erythroid cells to induced pluripotent stem cells

Niraj Bhatt, Rajib Ghosh, Sanchita Roy, Yongxing Gao, Mary Armanios, Linzhao Cheng, Sonia Franco

Research output: Contribution to journalArticle

Abstract

Biallelic mutations in ATM result in the neurodegenerative syndrome Ataxia-Telangiectasia, while ATM haploinsufficiency increases the risk of cancer and other diseases. Previous studies revealed low reprogramming efficiency from A-T and carrier fibroblasts, a barrier to iPS cell-based modeling and regeneration. Here, we tested the feasibility of employing circulating erythroid cells, a compartment no or minimally affected in A-T, for the generation of A-T and carrier iPS cells. Our results indicate that episomal expression of Yamanaka factors plus BCL-xL in erythroid cells results in highly efficient iPS cell production in feeder-free, xeno-free conditions. Moreover, A-T iPS cells generated with this protocol maintain long-term replicative potential, stable karyotypes, re-elongated telomeres and capability to differentiate along the neural lineage in vitro and to form teratomas in vivo. Finally, we find that haploinsufficiency for ATM does not limit reprogramming from human erythroid cells or in vivo teratoma formation in the mouse.

Original languageEnglish (US)
Pages (from-to)296-305
Number of pages10
JournalStem Cell Research
Volume17
Issue number2
DOIs
StatePublished - Sep 1 2016

    Fingerprint

Keywords

  • Ataxia-Telangiectasia
  • ATM
  • induced pluripotent stem cells
  • radiation
  • telomere
  • teratoma

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this