Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes

Luciano A. Moreira, Marten J. Edwards, Faisal Adhami, Nijole Jasinskiene, Anthony A. James, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on the characterization in transgenic Aedes aegypti of two mosquito gut-specific promoters. A 1.4-kb DNA fragment adjacent to the 5' end of the coding region of the Ae. aegypti carboxypeptidase (AeCP) gene and a corresponding 3.4-kb DNA fragment at the 5' end of the Anopheles gambiae carboxypeptidase (AgCP) gene were linked to a firefly luciferase reporter gene and introduced into the Ae. aegypti germ line by using Hermes and mariner (Mos1) transposons. Six independent transgenic lines were obtained with the AeCP construct and one with the AgCP construct. Luciferase mRNA and protein were abundantly expressed in the guts of transgenic mosquitoes in four of the six AeCP lines and in the AgCP line. Expression of the reporter gene was gut-specific and reached peak levels at about 24 h post-blood ingestion. The AeCP and AgCP promoters can be used to drive the expression of genes that hinder parasite development in the mosquito gut.

Original languageEnglish (US)
Pages (from-to)10895-10898
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number20
DOIs
StatePublished - Sep 26 2000

Keywords

  • Hermes
  • Malaria
  • Mariner
  • Promoter function

ASJC Scopus subject areas

  • General

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