Robust fluorescent labelling of micropipettes for use in fluorescence microscopy: application to the observation of a mosquito borne parasite infection

Amanda E. Balaban, Keir Neuman, Photini Sinnis, Robert S. Balaban

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to monitor micropipette injections with a high-resolution fluorescent microscope has utility for a variety of applications. Herein, different approaches were tested for creating broad-band fluorescently labelled glass micropipettes including: UV cured glass glues, baked glass enamel containing fluorescent dyes as well as nanodiamonds attached during pipette formation in the microforge. The most robust and simplest approach was to use labelled baked enamel on the exterior of the pipette. This approach was tested using pipettes designed to mimic a mosquito proboscis for the injection of the malaria parasite, Plasmodium spp., into the dermis of a living mouse ear. The pipette (∼30 micron diameter) was easily detected in the microscopy field of view and tolerated multiple insertions through the skin. This simple inexpensive approach to fluorescently labelling micropipettes will aid in the development of procedures under the fluorescent microscope.

Original languageEnglish (US)
Pages (from-to)78-84
Number of pages7
JournalJournal of Microscopy
Volume269
Issue number1
DOIs
StatePublished - Jan 2018

Keywords

  • 2-photon excitation
  • Intravital imaging
  • malaria
  • mouse
  • skin
  • sporozoites

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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