RNAi-mediated silencing of nuclear factor erythroid-2-related factor 2 gene expression in non-small cell lung cancer inhibits tumor growth and increases efficacy of chemotherapy

Anju Singh, Swetlana Boldin-Adamsky, Rajesh K. Thimmulappa, Srikanta K. Rath, Hagit Ashush, Jonathan Coulter, Amanda Blackford, Steven N. Goodman, Fred Bunz, Walter H. Watson, Edward Gabrielson, Elena Feinstein, Shyam Biswal

Research output: Contribution to journalArticle

Abstract

Nuclear factor erythroid-2-related factor 2 (Nrf2) is a redox-sensitive transcription factor that regulates the expression of electrophile and xenobiotic detoxification enzymes and efflux proteins, which confer cytoprotection against oxidative stress and apoptosis in normal cells. Loss of function mutations in the Nrf2 inhibitor, Kelch-like ECH-associated protein (Keap1), results in constitutive activation of Nrf2 function in non-small cell lung cancer. In this study, we show that constitutive activation of Nrf2 in lung cancer cells promotes tumorigenicity and contributes to chemoresistance by up-regulation of glutathione, thioredoxin, and the drug efflux pathways involved in detoxification of electrophiles and broad spectrum of drugs. RNAi-mediated reduction of Nrf2 expression in lung cancer cells induces generation of reactive oxygen species, suppresses tumor growth, and results in increased sensitivity to chemotherapeutic drug-induced cell death in vitro and in vivo. Inhibiting Nrf2 expression using naked siRNA duplexes in combination with carboplatin significantly inhibits tumor growth in a subcutaneous model of lung cancer. Thus, targeting Nrf2 activity in lung cancers, particularly those with Keap1 mutations, could be a promising strategy to inhibit tumor growth and circumvent chemoresistance.

Original languageEnglish (US)
Pages (from-to)7975-7984
Number of pages10
JournalCancer Research
Volume68
Issue number19
DOIs
StatePublished - Oct 1 2008

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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