RNAi libraries and kinetoplast DNA

P. T. Englund, E. E.C. Agbo, M. E. Lindsay, B. Liu, Y. Liu, S. A. Motyka, G. Yildirir, Z. Zhao

Research output: Contribution to journalArticle

Abstract

African trypanosomes have a remarkable mitochondrial DNA termed kDNA (kinetoplast DNA) that contains several thousands of topologically interlocked DNA rings. Because of its highly unusual structure, kDNA has a complex replication mechanism. Our approach to understanding this mechanism is to identify the proteins involved and to characterize their function. So far approx. 30 candidate proteins have been discovered and we predict that there are over 100. To identify genes for more kDNA replication proteins, we are using an RNA interference library, which is the first forward genetic approach used for these parasites.

Original languageEnglish (US)
Pages (from-to)1409-1412
Number of pages4
JournalBiochemical Society transactions
Volume33
Issue number6
DOIs
StatePublished - Dec 1 2005

Keywords

  • DNA replication
  • Kinetoplast DNA (kDNA)
  • Maxicircle replication
  • RNA interference (RNAi)
  • Trypanosoma brucei
  • Trypanosome

ASJC Scopus subject areas

  • Biochemistry

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  • Cite this

    Englund, P. T., Agbo, E. E. C., Lindsay, M. E., Liu, B., Liu, Y., Motyka, S. A., Yildirir, G., & Zhao, Z. (2005). RNAi libraries and kinetoplast DNA. Biochemical Society transactions, 33(6), 1409-1412. https://doi.org/10.1042/BST20051409