RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena

Yifan Liu, Sean Dixon Taverna, Tara L. Muratore, Jeffrey Shabanowitz, Donald F. Hunt, C. David Allis

Research output: Contribution to journalArticle

Abstract

Methylated H3K27 is an important mark for Polycomb group (PcG) protein-mediated transcriptional gene silencing (TGS) in multicellular eukaryotes. Here a Drosophila E(z) homolog, EZL1, is characterized in the ciliated protozoan Tetrahymena thermophila and is shown to be responsible for H3K27 methylation associated with developmentally regulated heterochromatin formation and DNA elimination. Importantly, Ezl1p-catalyzed H3K27 methylation occurs in an RNA interference (RNAi)-dependent manner. H3K27 methylation also regulates H3K9 methylation in these processes. Furthermore, an "effector" of programmed DNA elimination, the chromodomain protein Pdd1p, is shown to bind both K27- and K9-methylated H3. These studies provide a framework for an RNAi-dependent, Polycomb group protein-mediated heterochromatin formation pathway in Tetrahymena and underscore the connection between the two highly conserved machineries in eukaryotes.

Original languageEnglish (US)
Pages (from-to)1530-1545
Number of pages16
JournalGenes & development
Volume21
Issue number12
DOIs
StatePublished - Jun 15 2007
Externally publishedYes

Fingerprint

Tetrahymena
Heterochromatin
RNA Interference
Methylation
Polycomb-Group Proteins
DNA
Eukaryota
Tetrahymena thermophila
Gene Silencing
Drosophila
Proteins

Keywords

  • DNA elimination
  • H3K27 methylation
  • Heterochromatin
  • Polycomb group proteins
  • RNA interference

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena. / Liu, Yifan; Taverna, Sean Dixon; Muratore, Tara L.; Shabanowitz, Jeffrey; Hunt, Donald F.; Allis, C. David.

In: Genes & development, Vol. 21, No. 12, 15.06.2007, p. 1530-1545.

Research output: Contribution to journalArticle

Liu, Yifan ; Taverna, Sean Dixon ; Muratore, Tara L. ; Shabanowitz, Jeffrey ; Hunt, Donald F. ; Allis, C. David. / RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena. In: Genes & development. 2007 ; Vol. 21, No. 12. pp. 1530-1545.
@article{2bca5b2191f24464ac94db3f5aa9f513,
title = "RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena",
abstract = "Methylated H3K27 is an important mark for Polycomb group (PcG) protein-mediated transcriptional gene silencing (TGS) in multicellular eukaryotes. Here a Drosophila E(z) homolog, EZL1, is characterized in the ciliated protozoan Tetrahymena thermophila and is shown to be responsible for H3K27 methylation associated with developmentally regulated heterochromatin formation and DNA elimination. Importantly, Ezl1p-catalyzed H3K27 methylation occurs in an RNA interference (RNAi)-dependent manner. H3K27 methylation also regulates H3K9 methylation in these processes. Furthermore, an {"}effector{"} of programmed DNA elimination, the chromodomain protein Pdd1p, is shown to bind both K27- and K9-methylated H3. These studies provide a framework for an RNAi-dependent, Polycomb group protein-mediated heterochromatin formation pathway in Tetrahymena and underscore the connection between the two highly conserved machineries in eukaryotes.",
keywords = "DNA elimination, H3K27 methylation, Heterochromatin, Polycomb group proteins, RNA interference",
author = "Yifan Liu and Taverna, {Sean Dixon} and Muratore, {Tara L.} and Jeffrey Shabanowitz and Hunt, {Donald F.} and Allis, {C. David}",
year = "2007",
month = "6",
day = "15",
doi = "10.1101/gad.1544207",
language = "English (US)",
volume = "21",
pages = "1530--1545",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "12",

}

TY - JOUR

T1 - RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena

AU - Liu, Yifan

AU - Taverna, Sean Dixon

AU - Muratore, Tara L.

AU - Shabanowitz, Jeffrey

AU - Hunt, Donald F.

AU - Allis, C. David

PY - 2007/6/15

Y1 - 2007/6/15

N2 - Methylated H3K27 is an important mark for Polycomb group (PcG) protein-mediated transcriptional gene silencing (TGS) in multicellular eukaryotes. Here a Drosophila E(z) homolog, EZL1, is characterized in the ciliated protozoan Tetrahymena thermophila and is shown to be responsible for H3K27 methylation associated with developmentally regulated heterochromatin formation and DNA elimination. Importantly, Ezl1p-catalyzed H3K27 methylation occurs in an RNA interference (RNAi)-dependent manner. H3K27 methylation also regulates H3K9 methylation in these processes. Furthermore, an "effector" of programmed DNA elimination, the chromodomain protein Pdd1p, is shown to bind both K27- and K9-methylated H3. These studies provide a framework for an RNAi-dependent, Polycomb group protein-mediated heterochromatin formation pathway in Tetrahymena and underscore the connection between the two highly conserved machineries in eukaryotes.

AB - Methylated H3K27 is an important mark for Polycomb group (PcG) protein-mediated transcriptional gene silencing (TGS) in multicellular eukaryotes. Here a Drosophila E(z) homolog, EZL1, is characterized in the ciliated protozoan Tetrahymena thermophila and is shown to be responsible for H3K27 methylation associated with developmentally regulated heterochromatin formation and DNA elimination. Importantly, Ezl1p-catalyzed H3K27 methylation occurs in an RNA interference (RNAi)-dependent manner. H3K27 methylation also regulates H3K9 methylation in these processes. Furthermore, an "effector" of programmed DNA elimination, the chromodomain protein Pdd1p, is shown to bind both K27- and K9-methylated H3. These studies provide a framework for an RNAi-dependent, Polycomb group protein-mediated heterochromatin formation pathway in Tetrahymena and underscore the connection between the two highly conserved machineries in eukaryotes.

KW - DNA elimination

KW - H3K27 methylation

KW - Heterochromatin

KW - Polycomb group proteins

KW - RNA interference

UR - http://www.scopus.com/inward/record.url?scp=34250735582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250735582&partnerID=8YFLogxK

U2 - 10.1101/gad.1544207

DO - 10.1101/gad.1544207

M3 - Article

VL - 21

SP - 1530

EP - 1545

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 12

ER -