RNA-seq of spinal cord from nerve-injured rats after spinal cord stimulation

Kimberly E. Stephens, Zhiyong Chen, Eellan Sivanesan, Srinivasa Naga Raja, Bengt Linderoth, Sean Dixon Taverna, Yun Guan

Research output: Contribution to journalArticle

Abstract

Spinal cord stimulation has become an important modality in pain treatment especially for neuropathic pain conditions refractory to pharmacotherapy. However, the molecular control of inhibitory and excitatory mechanisms observed after spinal cord stimulation are poorly understood. Here, we used RNA-seq to identify differences in the expression of genes and gene networks in spinal cord tissue from nerve-injured rats with and without repetitive conventional spinal cord stimulation treatment. Five weeks after chronic constrictive injury to the left sciatic nerve, male and female rats were randomized to receive repetitive spinal cord stimulation or no treatment. Rats receiving spinal cord stimulation underwent epidural placement of a miniature stimulating electrode and received seven sessions of spinal cord stimulation (50 Hz, 80% motor threshold, 0.2 ms, constant current bipolar stimulation, 120 min/session) over four consecutive days. Within 2 h after the last spinal cord stimulation treatment, the L4-L6 spinal segments ipsilateral to the side of nerve injury were harvested and used to generate libraries for RNA-seq. Our RNA-seq data suggest further increases of many existing upregulated immune responses in chronic constrictive injury rats after repetitive spinal cord stimulation, including transcription of cell surface receptors and activation of non-neuronal cells. We also demonstrate that repetitive spinal cord stimulation represses transcription of several key synaptic signaling genes that encode scaffold proteins in the post-synaptic density. Our transcriptional studies suggest a potential relationship between specific genes and the therapeutic effects observed in patients undergoing conventional spinal cord stimulation after nerve injury. Furthermore, our results may help identify new therapeutic targets for improving the efficacy of conventional spinal cord stimulation and other chronic pain treatments.

Original languageEnglish (US)
JournalMolecular Pain
Volume14
DOIs
StatePublished - Dec 1 2018

Fingerprint

Spinal Cord Stimulation
Spinal Nerves
Spinal Cord
RNA
Wounds and Injuries
Therapeutics
Post-Synaptic Density
Nerve Tissue
Gene Regulatory Networks
Cell Surface Receptors
Neuralgia
Therapeutic Uses
Sciatic Nerve
Chronic Pain
Genes
Libraries
Electrodes

Keywords

  • gene expression
  • nerve injury
  • pain
  • RNA-seq
  • spinal cord
  • spinal cord stimulation

ASJC Scopus subject areas

  • Molecular Medicine
  • Cellular and Molecular Neuroscience
  • Anesthesiology and Pain Medicine

Cite this

RNA-seq of spinal cord from nerve-injured rats after spinal cord stimulation. / Stephens, Kimberly E.; Chen, Zhiyong; Sivanesan, Eellan; Raja, Srinivasa Naga; Linderoth, Bengt; Taverna, Sean Dixon; Guan, Yun.

In: Molecular Pain, Vol. 14, 01.12.2018.

Research output: Contribution to journalArticle

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