RNA polymerase II C-terminal domain required for enhancer-driven transcription

Hans Peter Gerber, Michael Hagmann, Katja Seipel, Oleg Georgiev, Marilyn A L West, Ying Litingtung, Walter Schaffner, Jeffry Lynn Corden

Research output: Contribution to journalArticle

Abstract

The RNA polymerase II carboxy-terminal domain (CTD) consists of tandem repeats of the sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser1-3. The CTD may participate in activated transcription through interaction with a high-molecular-weight mediator complex4-6. Such a role would be consistent with observations that some genes are preferentially sensitive to CTD mutations7,8. Here we investigate the function of the mouse RNA polymerase CTD in enhancer-driven transcription. Transcription by α-amanitin-resistant CTD-deletion mutants was tested by transient transfection of tissue culture cells in the presence of α-amanitin in order to inhibit endogenous RNA polymerase II. Removal of most of the CTD abolishes transcriptional activation by all enhancers tested, whereas transcription from promoters driven by Spl, a factor that typically activates housekeeping genes from positions proximal to the initiation sites, is not affected. These findings show that the CTD is essential in mediating 'enhancer'-type activation of mammalian transcription.

Original languageEnglish (US)
Pages (from-to)660-662
Number of pages3
JournalNature
Volume374
Issue number6523
StatePublished - 1995

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Amanitins
RNA Polymerase III
RNA Polymerase II
Transcriptional Activation
Tandem Repeat Sequences
Gene Order
Essential Genes
DNA-Directed RNA Polymerases
Transfection
Cell Culture Techniques
Molecular Weight
Genes

ASJC Scopus subject areas

  • General

Cite this

Gerber, H. P., Hagmann, M., Seipel, K., Georgiev, O., West, M. A. L., Litingtung, Y., ... Corden, J. L. (1995). RNA polymerase II C-terminal domain required for enhancer-driven transcription. Nature, 374(6523), 660-662.

RNA polymerase II C-terminal domain required for enhancer-driven transcription. / Gerber, Hans Peter; Hagmann, Michael; Seipel, Katja; Georgiev, Oleg; West, Marilyn A L; Litingtung, Ying; Schaffner, Walter; Corden, Jeffry Lynn.

In: Nature, Vol. 374, No. 6523, 1995, p. 660-662.

Research output: Contribution to journalArticle

Gerber, HP, Hagmann, M, Seipel, K, Georgiev, O, West, MAL, Litingtung, Y, Schaffner, W & Corden, JL 1995, 'RNA polymerase II C-terminal domain required for enhancer-driven transcription', Nature, vol. 374, no. 6523, pp. 660-662.
Gerber HP, Hagmann M, Seipel K, Georgiev O, West MAL, Litingtung Y et al. RNA polymerase II C-terminal domain required for enhancer-driven transcription. Nature. 1995;374(6523):660-662.
Gerber, Hans Peter ; Hagmann, Michael ; Seipel, Katja ; Georgiev, Oleg ; West, Marilyn A L ; Litingtung, Ying ; Schaffner, Walter ; Corden, Jeffry Lynn. / RNA polymerase II C-terminal domain required for enhancer-driven transcription. In: Nature. 1995 ; Vol. 374, No. 6523. pp. 660-662.
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AU - Litingtung, Ying

AU - Schaffner, Walter

AU - Corden, Jeffry Lynn

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