RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA and mRNA profiles in Drosophila ovaries

Li Zhu, Susan E. Liao, Yiwei Ai, Ryuya Fukunaga

Research output: Contribution to journalArticle

Abstract

RNA methyltransferases post-transcriptionally add methyl groups to RNAs, which can regulate their fates and functions. Human BCDIN3D (Bicoid interacting 3 domain containing RNA methyltransferase) has been reported to specifically methylate the 5′-monophosphates of pre-miR-145 and cytoplasmic tRNAHis. Methylation of the 5′-monophosphate of pre-miR-145 blocks its cleavage by the miRNA generating enzyme Dicer, preventing generation of miR-145. Elevated expression of BCDIN3D has been associated with poor prognosis in breast cancer. However, the biological functions of BCDIN3D and its orthologs remain unknown. Here we studied the biological and molecular functions of CG1239, a Drosophila ortholog of BCDIN3D. We found that ovary-specific knockdown of Drosophila BCDIN3D causes female sterility. High-throughput sequencing revealed that miRNA and mRNA profiles are dysregulated in BCDIN3D knockdown ovaries. Pathway analysis showed that many of the dysregulated genes are involved in metabolic processes, ribonucleoprotein complex regulation, and translational control. Our results reveal BCDIN3D's biological role in female fertility and its molecular role in defining miRNA and mRNA profiles in ovaries.

Original languageEnglish (US)
Article numbere0217603
JournalPloS one
Volume14
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

female fertility
methyltransferases
Methyltransferases
MicroRNAs
microRNA
Drosophila
Fertility
Ovary
RNA
Messenger RNA
RNA, Transfer, His
Ribonuclease III
Female Infertility
ribonucleoproteins
Methylation
Ribonucleoproteins
methylation
breast neoplasms
prognosis
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA and mRNA profiles in Drosophila ovaries. / Zhu, Li; Liao, Susan E.; Ai, Yiwei; Fukunaga, Ryuya.

In: PloS one, Vol. 14, No. 5, e0217603, 01.05.2019.

Research output: Contribution to journalArticle

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