RNA metabolism in rat intestinal mucosa of normal and vitamin A-deficient rats

L. De Luca, H. K. Kleinman, E. P. Little, G. Wolf

Research output: Contribution to journalArticle

Abstract

The decreased rate of leucine incorporation into leucyl-tRNA by the pH 5 enzyme fraction from vitamin A-deficient rat intestinal mucosa was investigated. When tRNA from either normal or vitamin A-deficient rat intestinal mucosa was added to the vitamin A-deficient pH 5 enzyme fraction, the yield of leucyl-tRNA rose to normal levels. Freon column chromatography of iso-accepting leucyl-tRNAs and threonyl-tRNAs failed to show any qualitative difference between normal and vitamin A-deficient iso-accepting species. Hence, a decrease in the amount of tRNA available for charging is responsible for the decrease in charging activity of the pH 5 enzyme fraction from vitamin A-deficient rat intestinal mucosa. RNA synthesis in the intestinal mucosa of normal and vitamin A-deficient rats was studied after intraperitoneal injection of either 14C-6-orotic acid or 3H-G-uridine. In three experiments the amount of label incorporated into all RNA fractions was 30-45% higher in the vitamin A-normal than the vitamin A-deficient rats. Measurements of uridine, UMP, and UDP pool sizes and their specific radioactivities 1 hr after injection of the label revealed that the difference in RNA synthesis between normal and vitamin A-deficient rat intestinal mucosa represented a true difference in the incorporation of the label into RNA and not a dilution effect on the label in deficient rats.

Original languageEnglish (US)
Pages (from-to)332-337
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume145
Issue number1
DOIs
StatePublished - Jul 1971
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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