RNA metabolism in rat intestinal mucosa of normal and vitamin A-deficient rats

Luigi M De Luca, H. K. Kleinman, E. P. Little, G. Wolf

Research output: Contribution to journalArticle

Abstract

The decreased rate of leucine incorporation into leucyl-tRNA by the pH 5 enzyme fraction from vitamin A-deficient rat intestinal mucosa was investigated. When tRNA from either normal or vitamin A-deficient rat intestinal mucosa was added to the vitamin A-deficient pH 5 enzyme fraction, the yield of leucyl-tRNA rose to normal levels. Freon column chromatography of iso-accepting leucyl-tRNAs and threonyl-tRNAs failed to show any qualitative difference between normal and vitamin A-deficient iso-accepting species. Hence, a decrease in the amount of tRNA available for charging is responsible for the decrease in charging activity of the pH 5 enzyme fraction from vitamin A-deficient rat intestinal mucosa. RNA synthesis in the intestinal mucosa of normal and vitamin A-deficient rats was studied after intraperitoneal injection of either 14C-6-orotic acid or 3H-G-uridine. In three experiments the amount of label incorporated into all RNA fractions was 30-45% higher in the vitamin A-normal than the vitamin A-deficient rats. Measurements of uridine, UMP, and UDP pool sizes and their specific radioactivities 1 hr after injection of the label revealed that the difference in RNA synthesis between normal and vitamin A-deficient rat intestinal mucosa represented a true difference in the incorporation of the label into RNA and not a dilution effect on the label in deficient rats.

Original languageEnglish (US)
Pages (from-to)332-337
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume145
Issue number1
DOIs
StatePublished - 1971
Externally publishedYes

Fingerprint

Intestinal Mucosa
Vitamin A
Metabolism
Rats
RNA
Transfer RNA
Labels
Uridine
Enzymes
Transfer RNA Aminoacylation
Chlorofluorocarbons
Orotic Acid
Uridine Monophosphate
Mucous Membrane
Column chromatography
Uridine Diphosphate
Radioactivity
Intraperitoneal Injections
Leucine
Dilution

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

RNA metabolism in rat intestinal mucosa of normal and vitamin A-deficient rats. / De Luca, Luigi M; Kleinman, H. K.; Little, E. P.; Wolf, G.

In: Archives of Biochemistry and Biophysics, Vol. 145, No. 1, 1971, p. 332-337.

Research output: Contribution to journalArticle

De Luca, Luigi M ; Kleinman, H. K. ; Little, E. P. ; Wolf, G. / RNA metabolism in rat intestinal mucosa of normal and vitamin A-deficient rats. In: Archives of Biochemistry and Biophysics. 1971 ; Vol. 145, No. 1. pp. 332-337.
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