RNA interference for analysis of gene function in trypanosomatids

Shawn A. Motyka, Paul T. Englund

Research output: Contribution to journalReview articlepeer-review

Abstract

Gene-specific silencing by RNA interference is a valuable tool for analysis of gene function in the protozoan parasite Trypanosoma brucei. The development of tetracycline-regulated vectors for production of double-stranded RNA has facilitated its widespread use. RNA interference provides a fast and efficient method for determining whether a gene is essential for growth and viability, reveals mechanistic information on gene function, and has greatly enhanced our understanding of complex biological processes. Finally, the creation of an RNA interference-based library has allowed, for the first time, an approach for conducting forward genetic experiments in this organism.

Original languageEnglish (US)
Pages (from-to)362-368
Number of pages7
JournalCurrent Opinion in Microbiology
Volume7
Issue number4
DOIs
StatePublished - Aug 2004

Keywords

  • RNA interference
  • RNAi
  • conA
  • concanavalin A
  • double-stranded RNA
  • dsRNA
  • kDNA
  • kinetoplast DNA
  • siRNA
  • small interfering RNA

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Infectious Diseases

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