RNA fluorescence in situ hybridization in cultured mammalian cells

Vidisha Tripathi, Jingyi Fei, Taekjip Ha, Kannanganattu V. Prasanth

Research output: Contribution to journalArticle

Abstract

It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

Original languageEnglish (US)
Pages (from-to)123-136
Number of pages14
JournalMethods in Molecular Biology
Volume1206
DOIs
StatePublished - Jan 1 2015

Keywords

  • Fluorescence
  • In situ hybridization
  • lncRNA
  • Long noncoding RNA
  • RNA-FISH

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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