RNA fluorescence in situ hybridization in cultured mammalian cells

Vidisha Tripathi, Jingyi Fei, Taekjip Ha, Kannanganattu V. Prasanth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

Original languageEnglish (US)
Title of host publicationRegulatory Non-Coding RNAs: Methods and Protocols
PublisherSpringer New York
Pages123-136
Number of pages14
ISBN (Electronic)9781493913695
ISBN (Print)9781493913688
DOIs
StatePublished - Sep 20 2014
Externally publishedYes

Fingerprint

Long Noncoding RNA
Fluorescence In Situ Hybridization
Cultured Cells
Fluorescence
Cells
RNA
Gene expression
Gene Expression

Keywords

  • Fluorescence
  • In situ hybridization
  • lncRNA
  • Long noncoding RNA
  • RNA-FISH

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tripathi, V., Fei, J., Ha, T., & Prasanth, K. V. (2014). RNA fluorescence in situ hybridization in cultured mammalian cells. In Regulatory Non-Coding RNAs: Methods and Protocols (pp. 123-136). Springer New York. https://doi.org/10.1007/978-1-4939-1369-5_11

RNA fluorescence in situ hybridization in cultured mammalian cells. / Tripathi, Vidisha; Fei, Jingyi; Ha, Taekjip; Prasanth, Kannanganattu V.

Regulatory Non-Coding RNAs: Methods and Protocols. Springer New York, 2014. p. 123-136.

Research output: Chapter in Book/Report/Conference proceedingChapter

Tripathi, V, Fei, J, Ha, T & Prasanth, KV 2014, RNA fluorescence in situ hybridization in cultured mammalian cells. in Regulatory Non-Coding RNAs: Methods and Protocols. Springer New York, pp. 123-136. https://doi.org/10.1007/978-1-4939-1369-5_11
Tripathi V, Fei J, Ha T, Prasanth KV. RNA fluorescence in situ hybridization in cultured mammalian cells. In Regulatory Non-Coding RNAs: Methods and Protocols. Springer New York. 2014. p. 123-136 https://doi.org/10.1007/978-1-4939-1369-5_11
Tripathi, Vidisha ; Fei, Jingyi ; Ha, Taekjip ; Prasanth, Kannanganattu V. / RNA fluorescence in situ hybridization in cultured mammalian cells. Regulatory Non-Coding RNAs: Methods and Protocols. Springer New York, 2014. pp. 123-136
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