RNA editing involves indiscriminate U changes throughout precisely defined editing domains

Carolyn J. Decker, Barbara Sollner-Webb

Research output: Contribution to journalArticlepeer-review

Abstract

RNA editing is the specific posttranscriptional insertion/deletion of U residues within trypanosomatid mitochondrial transcripts. We used a novel cDNA cloning scheme and analyzed partially edited COIII and CYb RNAs. Our major unanticipated findings are: First, editing appears strikingly indiscriminate within editing domains, inserting and deleting variable numbers of U residues at all sites, both those requiring editing and those that do not. Second, this incomplete editing occurs in precise domains, not in adjacent regions unedited in mature mRNA. Third, editing appears to commence prior to polyadenylation. We propose that within a region being edited, there are cycles of indiscriminate cleavage, U addition or deletion, and religation; sites that become correctly edited would be protected from further modification by duplexing with short RNAs complementary to the edited sequence.

Original languageEnglish (US)
Pages (from-to)1001-1011
Number of pages11
JournalCell
Volume61
Issue number6
DOIs
StatePublished - Jun 15 1990

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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