RNA-DNA hybridization in solution without DNA reannealing

Bert Vogelstein; David Gillespie

doi:10.1016/0006-291X(77)91500-5

RNA-DNA hybridization in solution without DNA reannealing

Research output: Contribution to journal › Article › peer-review

Abstract

In solutions containing 70% formamide hybridization of RNA to denatured DNA proceeded in the absence of DNA reannealing at temperatures permitting the optimum rate of formation of RNA-DNA hybrids. At low complementary DNA to RNA ratios the percentage of E. coli ribosomal RNA hybridized was substantially higher in 70% formamide than in solutions lacking formamide. The ability to perform RNA-DNA hybridization without competing DNA reannealing facilitates the description of the kinetics and stoichiometry of the reaction.

Original language	English (US)
Pages (from-to)	1127-1132
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	75
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(77)91500-5
State	Published - Apr 25 1977
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "RNA-DNA hybridization in solution without DNA reannealing",

abstract = "In solutions containing 70% formamide hybridization of RNA to denatured DNA proceeded in the absence of DNA reannealing at temperatures permitting the optimum rate of formation of RNA-DNA hybrids. At low complementary DNA to RNA ratios the percentage of E. coli ribosomal RNA hybridized was substantially higher in 70% formamide than in solutions lacking formamide. The ability to perform RNA-DNA hybridization without competing DNA reannealing facilitates the description of the kinetics and stoichiometry of the reaction.",

author = "Bert Vogelstein and David Gillespie",

year = "1977",

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doi = "10.1016/0006-291X(77)91500-5",

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AU - Gillespie, David

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AB - In solutions containing 70% formamide hybridization of RNA to denatured DNA proceeded in the absence of DNA reannealing at temperatures permitting the optimum rate of formation of RNA-DNA hybrids. At low complementary DNA to RNA ratios the percentage of E. coli ribosomal RNA hybridized was substantially higher in 70% formamide than in solutions lacking formamide. The ability to perform RNA-DNA hybridization without competing DNA reannealing facilitates the description of the kinetics and stoichiometry of the reaction.

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RNA-DNA hybridization in solution without DNA reannealing

Abstract

ASJC Scopus subject areas

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