Abstract
In solutions containing 70% formamide hybridization of RNA to denatured DNA proceeded in the absence of DNA reannealing at temperatures permitting the optimum rate of formation of RNA-DNA hybrids. At low complementary DNA to RNA ratios the percentage of E. coli ribosomal RNA hybridized was substantially higher in 70% formamide than in solutions lacking formamide. The ability to perform RNA-DNA hybridization without competing DNA reannealing facilitates the description of the kinetics and stoichiometry of the reaction.
Original language | English (US) |
---|---|
Pages (from-to) | 1127-1132 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 75 |
Issue number | 4 |
DOIs | |
State | Published - Apr 25 1977 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology