RNA-DNA hybridization in solution without DNA reannealing

Bert Vogelstein, David Gillespie

Research output: Contribution to journalArticle

Abstract

In solutions containing 70% formamide hybridization of RNA to denatured DNA proceeded in the absence of DNA reannealing at temperatures permitting the optimum rate of formation of RNA-DNA hybrids. At low complementary DNA to RNA ratios the percentage of E. coli ribosomal RNA hybridized was substantially higher in 70% formamide than in solutions lacking formamide. The ability to perform RNA-DNA hybridization without competing DNA reannealing facilitates the description of the kinetics and stoichiometry of the reaction.

Original languageEnglish (US)
Pages (from-to)1127-1132
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume75
Issue number4
DOIs
StatePublished - Apr 25 1977

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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