RNA-binding proteins that inhibit RNA virus infection

Jian Zhu, Kodetham Gopinath, Ayaluru Murali, Guanghui Yi, S. Diane Hayward, Heng Zhu, Cheng Kao

Research output: Contribution to journalArticle

Abstract

Arrays of >5,000 Saccharomyces cerevisiae proteins were screened to identify proteins that can preferentially bind a small RNA hairpin that contains a clamped adenine motif (CAM). A CAM is required for the replication of Brome Mosaic Virus (BMV), a plant-infecting RNA virus that can replicate in S. cerevisiae. Several hits were selected for further characterization in Nicotiana benthamiana. Pseudouridine Synthase 4 (Pus4) and the Actin Patch Protein 1 (App1) modestly reduced BMV genomic plus-strand RNA accumulation, but dramatically inhibited BMV systemic spread in plants. Pus4 also prevented the encapsidation of a BMV RNA in plants and the reassembly of BMV virions in vitro. These results demonstrate the feasibility of using proteome arrays to identify specific RNA-binding proteins for antiviral activities. Furthermore, the effects of Pus4 suggest that the CAM-containing RNA motif provides a regulatory link between RNA replication and encapsidation.

Original languageEnglish (US)
Pages (from-to)3129-3134
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number9
DOIs
StatePublished - Feb 27 2007

Keywords

  • Brome mosaic virus
  • Protein-RNA interaction
  • Pseudouridine synthase 4
  • Viral RNA replication
  • Yeast proteome chip

ASJC Scopus subject areas

  • General

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