RNA-binding protein AUF1 represses Dicer expression

Kotb Abdelmohsen, Kumiko Tominaga-Yamanaka, Subramanya Srikantan, Je Hyun Yoon, Min Ju Kang, Myriam Gorospe

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNA (miRNA) biogenesis is tightly regulated by numerous proteins. Among them, Dicer is required for the processing of the precursor (pre-)miRNAs into the mature miRNA. Despite its critical function, the mechanisms that regulate Dicer expression are not well understood. Here we report that the RNA-binding protein (RBP) AUF1 (AU-binding factor 1) associates with the endogenous DICER1 mRNA and can interact with several segments of DICER1 mRNA within the coding region (CR) and the 3′-untranslated region (UTR). Through these interactions, AUF1 lowered DICER1 mRNA stability, since silencing AUF1 lengthened DICER1 mRNA half-life and increased Dicer expression, while overexpressing AUF1 lowered DICER1 mRNA and Dicer protein levels. Given that Dicer is necessary for the synthesis of mature miRNAs, the lowering of Dicer levels by AUF1 diminished the levels of miRNAs tested, but not the levels of the corresponding pre-miRNAs. In summary, AUF1 suppresses miRNA production by reducing Dicer production.

Original languageEnglish (US)
Pages (from-to)11531-11544
Number of pages14
JournalNucleic acids research
Volume40
Issue number22
DOIs
StatePublished - Dec 2012

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'RNA-binding protein AUF1 represses Dicer expression'. Together they form a unique fingerprint.

Cite this