RNA as a flexible scaffold for proteins

Yeast telomerase and beyond

David Zappulla, T. R. Cech

Research output: Contribution to journalArticle

Abstract

Yeast telomerase, the enzyme that adds a repeated DNA sequence to the ends of the chromosomes, consists of a 1157-nucleotide RNA (TLC1) plus several protein subunits: the telomerase reverse transcriptase Est2p, the regulatory subunit Est1p, the nonhomologous end-joining heterodimer Ku, and the seven Sm proteins involved in ribonucleoprotein (RNP) maturation. The RNA subunit provides the template for telomeric DNA synthesis. In addition, we have reported evidence that it serves as a flexible scaffold to tether the proteins into the complex. More generally, we consider the possibility that RNPs may be considered in three structural categories: (1) those that have specific structures determined in large part by the RNA, including RNase P, other ribozyme - protein complexes, and the ribosome; (2) those that have specific structures determined in large part by proteins, including many small nuclear RNPs (snRNPs) and small nucleolar RNPs (snoRNPs); and (3) flexible scaffolds, with no specific structure of the RNP as a whole, as exemplified by yeast telomerase. Other candidates for flexible scaffold structures are other telomerases, viral IRES (internal ribosome entry site) elements, tmRNA (transfer-messenger RNA), the SRP (signal recognition particle), and Xist and roX1 RNAs that alter chromatin structure to achieve dosage compensation.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume71
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Fingerprint

Fungal Proteins
Telomerase
Scaffolds
RNA
Ribonucleoproteins
Yeast
Proteins
Yeasts
Signal Recognition Particle
Ribonuclease P
Small Nuclear Ribonucleoproteins
Catalytic RNA
DNA sequences
Protein Subunits
Chromosomes
Transfer RNA
Ribosomes
Joining
Chromatin
Nucleotides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

RNA as a flexible scaffold for proteins : Yeast telomerase and beyond. / Zappulla, David; Cech, T. R.

In: Cold Spring Harbor Symposia on Quantitative Biology, Vol. 71, 01.12.2006, p. 217-224.

Research output: Contribution to journalArticle

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