Risk of human immunodeficiency virus (HIV-1) infection among laboratory workers

Stanley H. Weiss, James J. Goedert, Suzanne Gartner, Mikulas Popovic, David Waters, Phillip Markham, Fulvia Di Marzo Veronese, Mitchell H. Gail, W. Emmett Barkley, Joseph Gibbons, Fred A. Gill, Michael Leuther, George M. Shaw, Robert C. Gallo, William A. Blattner

Research output: Contribution to journalArticle

Abstract

In a prospective cohort study of 265 laboratory and affiliated workers, one individual with no recognized risk factors for human immunodeficiency virus type 1 (HIV-1) infection was HIV-1 seropositive at the time of entry into the study. Molecular analyses of two HIV-1 isolates derived in two independent laboratories from a blood sample from this worker showed that die isolates were indistinguishable from a genotypic form of HIV-1 present in the H9/HTLV-III B cell line. Exposure to this strain of virus most probably occurred during work with concentrated virus or culture fluids from virus-producing cell lutes under standard Biosafety Level 3 containment. Although no specific incident leading to this infection has been identified, undetected skin contact with virus culture supernatant might have occurred. This worker was the only one found to be positive among the subgroup of 99 workers who shared a work environment involving exposure to concentrated virus. The incidence rate of 0.48 per 100 person-years exposure indicates that prolonged laboratory exposure to concentrated virus is associated with some risk of HIV-1 infection, which is comparable to the risk for health care workers experiencing a needle stick exposure. While none of the ten workers with parenteral exposure to HIV-1 in this cohort became infected, a worker in another laboratory did seroconvert following an injury with a potentially contaminated needle. Strict Biosafety Levd 3 containment and practices should be followed when working with concentrated HIV-1 preparations, and further refinement of the procedures may be necessary.

Original languageEnglish (US)
Pages (from-to)68-71
Number of pages4
JournalScience
Volume239
Issue number4835
StatePublished - 1988
Externally publishedYes

Fingerprint

Virus Diseases
HIV-1
HIV
Viruses
Needlestick Injuries
Needles
B-Lymphocytes
Cohort Studies
Prospective Studies
Delivery of Health Care
Cell Line
Skin
Incidence
Wounds and Injuries
Infection

ASJC Scopus subject areas

  • General

Cite this

Weiss, S. H., Goedert, J. J., Gartner, S., Popovic, M., Waters, D., Markham, P., ... Blattner, W. A. (1988). Risk of human immunodeficiency virus (HIV-1) infection among laboratory workers. Science, 239(4835), 68-71.

Risk of human immunodeficiency virus (HIV-1) infection among laboratory workers. / Weiss, Stanley H.; Goedert, James J.; Gartner, Suzanne; Popovic, Mikulas; Waters, David; Markham, Phillip; Di Marzo Veronese, Fulvia; Gail, Mitchell H.; Barkley, W. Emmett; Gibbons, Joseph; Gill, Fred A.; Leuther, Michael; Shaw, George M.; Gallo, Robert C.; Blattner, William A.

In: Science, Vol. 239, No. 4835, 1988, p. 68-71.

Research output: Contribution to journalArticle

Weiss, SH, Goedert, JJ, Gartner, S, Popovic, M, Waters, D, Markham, P, Di Marzo Veronese, F, Gail, MH, Barkley, WE, Gibbons, J, Gill, FA, Leuther, M, Shaw, GM, Gallo, RC & Blattner, WA 1988, 'Risk of human immunodeficiency virus (HIV-1) infection among laboratory workers', Science, vol. 239, no. 4835, pp. 68-71.
Weiss SH, Goedert JJ, Gartner S, Popovic M, Waters D, Markham P et al. Risk of human immunodeficiency virus (HIV-1) infection among laboratory workers. Science. 1988;239(4835):68-71.
Weiss, Stanley H. ; Goedert, James J. ; Gartner, Suzanne ; Popovic, Mikulas ; Waters, David ; Markham, Phillip ; Di Marzo Veronese, Fulvia ; Gail, Mitchell H. ; Barkley, W. Emmett ; Gibbons, Joseph ; Gill, Fred A. ; Leuther, Michael ; Shaw, George M. ; Gallo, Robert C. ; Blattner, William A. / Risk of human immunodeficiency virus (HIV-1) infection among laboratory workers. In: Science. 1988 ; Vol. 239, No. 4835. pp. 68-71.
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