Ribonucleoprotein-masked nicks at 50-kbp intervals in the eukaryotic genomic DNA

Lóránt Székvölgyi, Zsuzsa Rákosy, Bálint L. Bálint, Endre Kókai, László Imre, György Vereb, Zsolt Bacsó, Katalin Goda, Sándor Varga, Margit Balázs, Viktor Dombrádi, Laszlo Nagy, Gábor Szabó

Research output: Contribution to journalArticle

Abstract

By using a microscopic approach, field inversion single-cell gel electrophoresis, we show that preformed single-strand discontinuities are present in the chromatin of resting and proliferating mammalian and yeast cells. These single-strand breaks are primarily nicks positioned at ≈50-kbp intervals throughout the entire genome that could be efficiently labeled in situ by DNA polymerase I holoenzyme but not by Klenow fragment and terminal transferase unless after ribonucleolytic treatments. The RNA molecules involved appear to comprise R-loops, recognized by the S9.6 RNA/DNA hybrid-specific antibody. By using the breakpoint cluster region of the Mixed Lineage Leukemia (MLL) gene as a model, we have found that the number of manifest nicks detected by FISH performed after field inversion single-cell gel electrophoresis depends on epigenetic context, but the difference between germ-line and translocated MLL alleles is abolished by protease treatment. Our data imply that the double-stranded genomic DNA is composed of contiguous rather than continuous single strands and reveal an aspect of higher-order chromatin organization with ribonucleoprotein-associated persistent nicks defining ≈50-kbp domains.

Original languageEnglish (US)
Pages (from-to)14964-14969
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number38
DOIs
StatePublished - Sep 18 2007
Externally publishedYes

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DNA Polymerase I
Ribonucleoproteins
Comet Assay
Chromatin
Leukemia
RNA
Holoenzymes
DNA
Transferases
Epigenomics
Germ Cells
Peptide Hydrolases
Yeasts
Alleles
Genome
Antibodies
Genes

Keywords

  • Chromatin loop
  • RNA/DNA hybrid
  • Translocation

ASJC Scopus subject areas

  • General

Cite this

Ribonucleoprotein-masked nicks at 50-kbp intervals in the eukaryotic genomic DNA. / Székvölgyi, Lóránt; Rákosy, Zsuzsa; Bálint, Bálint L.; Kókai, Endre; Imre, László; Vereb, György; Bacsó, Zsolt; Goda, Katalin; Varga, Sándor; Balázs, Margit; Dombrádi, Viktor; Nagy, Laszlo; Szabó, Gábor.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 38, 18.09.2007, p. 14964-14969.

Research output: Contribution to journalArticle

Székvölgyi, L, Rákosy, Z, Bálint, BL, Kókai, E, Imre, L, Vereb, G, Bacsó, Z, Goda, K, Varga, S, Balázs, M, Dombrádi, V, Nagy, L & Szabó, G 2007, 'Ribonucleoprotein-masked nicks at 50-kbp intervals in the eukaryotic genomic DNA', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 38, pp. 14964-14969. https://doi.org/10.1073/pnas.0702269104
Székvölgyi, Lóránt ; Rákosy, Zsuzsa ; Bálint, Bálint L. ; Kókai, Endre ; Imre, László ; Vereb, György ; Bacsó, Zsolt ; Goda, Katalin ; Varga, Sándor ; Balázs, Margit ; Dombrádi, Viktor ; Nagy, Laszlo ; Szabó, Gábor. / Ribonucleoprotein-masked nicks at 50-kbp intervals in the eukaryotic genomic DNA. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 38. pp. 14964-14969.
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