Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila

Seung Jae Lee, Hong Xu, Craig Montell

Research output: Contribution to journalArticle

Abstract

A feature shared between Drosophila rhodopsin and nearly all other G protein-coupled receptors is agonist-dependent protein phosphorylation. Despite extensive analyses of Drosophila photo-transduction, the identity and function of the rhodopsin kinase (RK) have been elusive. Here, we provide evidence that G protein-coupled receptor kinase 1 (GPRK1), which is most similar to the β-adrenergic receptor kinases, G protein-coupled receptor kinase 2 (GRK2) and GRK3, is the fly RK. We show that GPRKI is enriched in photoreceptor cells, associates with the major Drosophila rhodopsin, Rhl, and phosphorylates the receptor. As is the case with mammalian GRK2 and GRK3, Drosophila GPRK1 includes a C-terminal pleckstrin homology domain, which binds to phosphoinositides and the Gβγ subunit. To address the role of GPRK1, we generated transgenic flies that expressed higher and lower levels of RK activity. Those flies with depressed levels of RK activity displayed a light response with a much larger amplitude than WT. Conversely, the amplitude of the light response was greatly suppressed in transgenic flies expressing abnormally high levels of RK activity. These data point to an evolutionarily conserved role for GPRK1 in modulating the amplitude of the visual response.

Original languageEnglish (US)
Pages (from-to)11874-11879
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number32
DOIs
StatePublished - Aug 10 2004

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G-Protein-Coupled Receptor Kinase 1
Drosophila
Diptera
G-Protein-Coupled Receptor Kinase 2
Rhodopsin
Light
Photoreceptor Cells
G-Protein-Coupled Receptors
Phosphatidylinositols
Adrenergic Receptors
Phosphotransferases
Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila. / Lee, Seung Jae; Xu, Hong; Montell, Craig.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 32, 10.08.2004, p. 11874-11879.

Research output: Contribution to journalArticle

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