Rho1 directs formin-mediated actin ring assembly during budding yeast cytokinesis

Nicola Tolliday, Lynn VerPlank, Rong Li

Research output: Contribution to journalArticle

Abstract

In eukaryotic cells, dynamic rearrangement of the actin cytoskeleton is critical for cell division. In the yeast Saccharomyces cerevisiae, three main structures constitute the actin cytoskeleton: cortical actin patches, cytoplasmic actin cables, and the actin-based cytokinetic ring [1-4]. The conserved Arp2/3 complex and a WASP-family protein mediate actin patch formation, whereas the yeast formins (Bni1 and Bnr1) promote assembly of actin cables [5-9]. However, the mechanism of actin ring formation is currently unclear. Here, we show that actin filaments are required for cytokinesis in S. cerevisiae, and that the actin ring is a highly dynamic structure that undergoes constant turnover. Assembly of the actin ring requires the formin-like proteins and profilin, but is not Arp2/3-mediated. Furthermore, the formin-dependent actin ring assembly pathway is regulated by the Rho-type GTPase Rho1 but not Cdc42. Finally, we show that the formins are not required for localization of Cyk1/Iqg1, an IQGAP-like protein previously shown to be required for actin ring formation, suggesting that formin-like proteins and Cyk1 act synergistically but independently in assembly of the actin ring.

Original languageEnglish (US)
Pages (from-to)1864-1870
Number of pages7
JournalCurrent Biology
Volume12
Issue number21
DOIs
StatePublished - Oct 29 2002
Externally publishedYes

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Saccharomycetales
Cytokinesis
cytokinesis
Yeast
actin
Actins
yeasts
microfilaments
Actin Cytoskeleton
Saccharomyces cerevisiae
Wiskott-Aldrich Syndrome Protein Family
proteins
Yeasts
Actin-Related Protein 2-3 Complex
Profilins
Cables
rho GTP-Binding Proteins
guanosinetriphosphatase
Eukaryotic Cells
Cell Division

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Rho1 directs formin-mediated actin ring assembly during budding yeast cytokinesis. / Tolliday, Nicola; VerPlank, Lynn; Li, Rong.

In: Current Biology, Vol. 12, No. 21, 29.10.2002, p. 1864-1870.

Research output: Contribution to journalArticle

Tolliday, Nicola ; VerPlank, Lynn ; Li, Rong. / Rho1 directs formin-mediated actin ring assembly during budding yeast cytokinesis. In: Current Biology. 2002 ; Vol. 12, No. 21. pp. 1864-1870.
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