Rhinovirus stimulation of lnterleukin-6 in vivo and in vitro: Evidence for nuclear factor κB-dependent transcriptional activation

Zhou Zhu, Weiliang Tang, Anuradha Ray, Yang Wu, Oskar Einarsson, Marie L. Landry, Jack Gwaltney, Jack A. Elias

Research output: Contribution to journalArticle

Abstract

To further understand the biology of rhinovirus (RV), we determined whether IL-6 was produced during RV infections and characterized the mechanism by which RV stimulates lung cell IL-6 production. In contrast to normals and minimally symptomatic volunteers, IL-6 was detected in the nasal washings from patients who developed colds after RV challenge. RV14 and RV1A, major and minor receptor group RVs, respectively, were potent stimulators of IL-6 protein production in vitro. These effects were associated with significant increases in IL-6 mRNA accumulation and gene transcription. RV was also a potent stimulator of IL-6 promoter-driven luciferase activity. This stimulation was modestly decreased by mutation of the nuclear factor (NF) - IL-6 site and abrogated by mutation of the NF-κB site in this promoter. An NF-κB-DNA binding activity, mediated by p65, p50, and p52 NF-κB moieties, was rapidly induced in RV-infected cells. Activator protein 1-DNA binding was not similarly altered. These studies demonstrate that IL-6 is produced during symptomatic RV infections, that RVs are potent stimulators of IL-6 elaboration, and that RV stimulation of IL-6 production is mediated by an NF-κB-dependent transcriptional stimulation pathway. IL-6 may play an important role in the pathogenesis of RV infection, and NF-κB activation is likely to be an important event in RV-induced pathologies.

Original languageEnglish (US)
Pages (from-to)421-430
Number of pages10
JournalJournal of Clinical Investigation
Volume97
Issue number2
StatePublished - Jan 15 1996
Externally publishedYes

Fingerprint

Rhinovirus
Transcriptional Activation
Interleukin-6
In Vitro Techniques
Infection
Nasal Lavage Fluid
B-Form DNA
Mutation
Transcription Factor AP-1
Luciferases
Volunteers

Keywords

  • Epithelial cell
  • Fibroblast
  • NF-IL-6
  • Upper respiratory tract infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Zhu, Z., Tang, W., Ray, A., Wu, Y., Einarsson, O., Landry, M. L., ... Elias, J. A. (1996). Rhinovirus stimulation of lnterleukin-6 in vivo and in vitro: Evidence for nuclear factor κB-dependent transcriptional activation. Journal of Clinical Investigation, 97(2), 421-430.

Rhinovirus stimulation of lnterleukin-6 in vivo and in vitro : Evidence for nuclear factor κB-dependent transcriptional activation. / Zhu, Zhou; Tang, Weiliang; Ray, Anuradha; Wu, Yang; Einarsson, Oskar; Landry, Marie L.; Gwaltney, Jack; Elias, Jack A.

In: Journal of Clinical Investigation, Vol. 97, No. 2, 15.01.1996, p. 421-430.

Research output: Contribution to journalArticle

Zhu, Z, Tang, W, Ray, A, Wu, Y, Einarsson, O, Landry, ML, Gwaltney, J & Elias, JA 1996, 'Rhinovirus stimulation of lnterleukin-6 in vivo and in vitro: Evidence for nuclear factor κB-dependent transcriptional activation', Journal of Clinical Investigation, vol. 97, no. 2, pp. 421-430.
Zhu, Zhou ; Tang, Weiliang ; Ray, Anuradha ; Wu, Yang ; Einarsson, Oskar ; Landry, Marie L. ; Gwaltney, Jack ; Elias, Jack A. / Rhinovirus stimulation of lnterleukin-6 in vivo and in vitro : Evidence for nuclear factor κB-dependent transcriptional activation. In: Journal of Clinical Investigation. 1996 ; Vol. 97, No. 2. pp. 421-430.
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