Rhenium-188 for inhibition of human aortic smooth muscle cell proliferation

Jakub Wiskirchen, Helmut Dittmann, Rainer Kehlbach, Jens Vogel-Claussen, Regina Gebert, Bernhard M. Dohmen, Wolfgang Schöber, Roland Bares, H. Peter Rodemann, Claus D. Claussen, Stephan H. Duda

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To evaluate dose-dependent growth-modulating effects of the β-γ emitter Rhenium-188 on cultured human aortic smooth muscle cells (haSMC). Methods and Materials: HaSMC were plated in 25 cm2 flasks. Two days after plating, cells were incubated with the Re-188 (beta Emax 2.12 MeV, tissue rangemax < 10 mm, T1/2 17 h) for five days. The doses administered were 0.2 Gy, 1, 4, 6, 8, 16, and 32 Gy. After five days, the radionuclide was removed. Cell growth, cell cycle distribution, and clonogenic activity were analyzed for the following 25 days. Results: The 0.2 and 1 Gy groups did not show relevant growth-inhibiting effects compared to the control groups. The 4 to 32 Gy groups presented dose-dependent growth inhibition, with a complete growth arrest of the 16 and 32 Gy groups. Clonogenic activity of the smooth muscle cell was strongly inhibited from doses ≥8 Gy. Flow cytometry showed a lasting dose-dependent G2/M phase block. Conclusion: Smooth muscle cell (SMC) growth can be controlled effectively with Re-188 for at least 25 days after radiation in vitro. As the first four weeks after arterial angioplasty are crucial concerning neointimal formation, Re-188 may be a valuable radionuclide to inhibit restenosis after arterial angioplasty.

Original languageEnglish (US)
Pages (from-to)809-815
Number of pages7
JournalInternational Journal of Radiation Oncology Biology Physics
Volume49
Issue number3
DOIs
StatePublished - Mar 1 2001

Keywords

  • Arteries-angioplasty
  • Arteries-radiation
  • Arteries-restenosis

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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