Rewriting the blueprint of life by synthetic genomics and genome engineering

Narayana Annaluru, Sivaprakash Ramalingam, Srinivasan Chandrasegaran

Research output: Contribution to journalReview articlepeer-review

Abstract

Advances in DNA synthesis and assembly methods over the past decade have made it possible to construct genome-size fragments from oligonucleotides. Early work focused on synthesis of small viral genomes, followed by hierarchical synthesis of wild-type bacterial genomes and subsequently on transplantation of synthesized bacterial genomes into closely related recipient strains. More recently, a synthetic designer version of yeast Saccharomyces cerevisiae chromosome III has been generated, with numerous changes from the wild-type sequence without having an impact on cell fitness and phenotype, suggesting plasticity of the yeast genome. A project to generate the first synthetic yeast genome - the Sc2.0 Project - is currently underway.

Original languageEnglish (US)
Article number125
JournalGenome biology
Volume16
Issue number1
DOIs
StatePublished - Jun 16 2015

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

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