Rewriting the blueprint of life by synthetic genomics and genome engineering

Narayana Annaluru, Sivaprakash Ramalingam, Srinivasan Chandrasegaran

Research output: Contribution to journalArticle

Abstract

Advances in DNA synthesis and assembly methods over the past decade have made it possible to construct genome-size fragments from oligonucleotides. Early work focused on synthesis of small viral genomes, followed by hierarchical synthesis of wild-type bacterial genomes and subsequently on transplantation of synthesized bacterial genomes into closely related recipient strains. More recently, a synthetic designer version of yeast Saccharomyces cerevisiae chromosome III has been generated, with numerous changes from the wild-type sequence without having an impact on cell fitness and phenotype, suggesting plasticity of the yeast genome. A project to generate the first synthetic yeast genome - the Sc2.0 Project - is currently underway.

Original languageEnglish (US)
Article number125
JournalGenome Biology
Volume16
Issue number1
DOIs
StatePublished - Jun 16 2015

Fingerprint

Genomics
Bacterial Genomes
genomics
engineering
genome
Yeasts
Genome
yeast
Genome Size
Viral Genome
yeasts
Oligonucleotides
synthesis
Saccharomyces cerevisiae
Chromosomes
Transplantation
Phenotype
transplantation
DNA
oligonucleotides

ASJC Scopus subject areas

  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Rewriting the blueprint of life by synthetic genomics and genome engineering. / Annaluru, Narayana; Ramalingam, Sivaprakash; Chandrasegaran, Srinivasan.

In: Genome Biology, Vol. 16, No. 1, 125, 16.06.2015.

Research output: Contribution to journalArticle

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