@article{48b31b91072742849d04cf37e729ee9c,
title = "Revisiting Extracellular RNA Release, Processing, and Function",
abstract = "It is assumed that RNAs enriched in extracellular samples were selected for release by their parental cells. However, recent descriptions of extracellular RNA (exRNA) biogenesis and their differential stabilities question this assumption, as they could produce identical outcomes. Here, we share our opinion about the importance of considering both selective and nonselective mechanisms for RNA release into the extracellular environment. In doing so, we provide new perspectives on RNA-mediated intercellular communication, including an analogy to communication through social media. We also argue that technical limitations have restricted the study of some of the most abundant exRNAs, both inside and outside extracellular vesicles (EVs). These RNAs may be better positioned to induce a response in recipient cells compared with low abundance miRNAs.",
keywords = "RNA secretion, exosomes, extracellular RNAs, liquid biopsy, microvesicles",
author = "Tosar, {Juan Pablo} and Kenneth Witwer and Alfonso Cayota",
note = "Funding Information: This work was inspired by fruitful discussions with Louise Laurent, Amy Buck, and James Patton during the Panel on Extracellular RNAs at ISEV2020 (annual meeting of the International Society for Extracellular Vesicles). The concept of extracellular dark matter was introduced by L.L. during this panel. Funding for this work was received from ANII, Uruguay (FCE_3_2018_1_148745) and from the National Institutes of Health (NIH) (UG3CA241694, supported by the NIH Common Fund, through the Office of Strategic Coordination/Office of the NIH Director). J.P.T. and A.C. are members and receive funding from PEDECIBA, ANII, and UdelaR (Uruguay). Funding Information: Funding for this work was received from ANII, Uruguay ( FCE_3_2018_1_148745 ) and from the National Institutes of Health (NIH) ( UG3CA241694 , supported by the NIH Common Fund, through the Office of Strategic Coordination / Office of the NIH Director ). J.P.T. and A.C. are members and receive funding from PEDECIBA, ANII, and UdelaR (Uruguay). Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",
year = "2021",
month = jun,
doi = "10.1016/j.tibs.2020.12.008",
language = "English (US)",
volume = "46",
pages = "438--445",
journal = "Trends in Biochemical Sciences",
issn = "0376-5067",
publisher = "Elsevier Limited",
number = "6",
}